Linked Life Data

9529137

Source:http://linkedlifedata.com/resource/pubmed/id/9529137

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1998-4-16
pubmed:abstractText
Despite the importance of bone marrow stromal cells in hemopoiesis, the profile of surface molecule expression is relatively poorly understood. Mice were immunized with cultured human bone marrow stromal cells in order to raise monoclonal antibodies to novel cell surface molecules, which might be involved in interactions with hemopoietic cells. Three antibodies, WM85, CC9 and EB4 were produced, and were found to identify a 100-110 kDa antigen on bone marrow fibroblasts. Molecular cloning revealed the molecule to be MUC18 (CD146), a member of the immunoglobulin superfamily, previously described as a marker of metastatic melanoma. In addition to the expected expression on melanoma cell lines and endothelial cells, a number of human leukemic cell lines were found to express MUC18, including all six T leukemia lines tested, one of five B lineage lines and one of four myeloid lines. Analysis of bone marrow samples from patients revealed positivity in 20% of B lineage ALL (n = 20), one of three T-ALL, 15% of AML (n = 13) and 43% of various B lymphoproliferative disorders (n = 7). No apparent reactivity was observed with mononuclear cells from normal peripheral blood or bone marrow, including candidate hemopoietic stem cells characterized by their expression of the CD34 antigen. However, positive selection of bone marrow mononuclear cells labeled with MUC18 antibody revealed a rare subpopulation (<1%) containing more than 90% of the stromal precursors identified in fibroblast colony-forming assays. The structure and tissue distribution of MUC18 suggest a functional role in regulation of hemopoiesis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0887-6924
pubmed:author
pubmed:issnType
Print
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
414-21
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:9529137-Animals, pubmed-meshheading:9529137-Antibodies, Monoclonal, pubmed-meshheading:9529137-Antigens, CD, pubmed-meshheading:9529137-Antigens, CD146, pubmed-meshheading:9529137-Bone Marrow Cells, pubmed-meshheading:9529137-Cells, Cultured, pubmed-meshheading:9529137-Cloning, Molecular, pubmed-meshheading:9529137-Endothelium, Vascular, pubmed-meshheading:9529137-Female, pubmed-meshheading:9529137-Hematologic Neoplasms, pubmed-meshheading:9529137-Humans, pubmed-meshheading:9529137-Leukemia, pubmed-meshheading:9529137-Leukemia, B-Cell, pubmed-meshheading:9529137-Leukemia, Myeloid, pubmed-meshheading:9529137-Leukemia, T-Cell, pubmed-meshheading:9529137-Melanoma, pubmed-meshheading:9529137-Membrane Glycoproteins, pubmed-meshheading:9529137-Mice, pubmed-meshheading:9529137-Mice, Inbred BALB C, pubmed-meshheading:9529137-Neural Cell Adhesion Molecules, pubmed-meshheading:9529137-Recombinant Proteins, pubmed-meshheading:9529137-Reference Values, pubmed-meshheading:9529137-Stromal Cells, pubmed-meshheading:9529137-Tumor Cells, Cultured, pubmed-meshheading:9529137-Tumor Markers, Biological
pubmed:year
1998
pubmed:articleTitle
MUC18, a member of the immunoglobulin superfamily, is expressed on bone marrow fibroblasts and a subset of hematological malignancies.
pubmed:affiliation
Department of Haematology, Westmead Hospital, New South Wales, Australia.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't