Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1998-5-21
pubmed:abstractText
Zeta-Crystallin is a taxon-specific crystallin, an enzyme which has undergone direct gene recruitment as a structural component of the guinea pig lens through a Pax6-dependent mechanism. Tissue specificity arises through a combination of effects involving three sites in the lens promoter. The Pax6 site (ZPE) itself shows specificity for an isoform of Pax6 preferentially expressed in lens cells. High-level expression of the promoter requires a second site, identical to an alphaCE2 site or half Maf response element (MARE), adjacent to the Pax6 site. A promoter fragment containing Pax6 and MARE sites gives lens-preferred induction of a heterologous promoter. Complexes binding the MARE in lens nuclear extracts are antigenically related to Nrl, and cotransfection with Nrl elevates zeta-crystallin promoter activity in lens cells. A truncated zeta promoter containing Nrl-MARE and Pax6 sites has a high level of expression in lens cells in transgenic mice but is also active in the brain. Suppression of the promoter in the brain requires sequences between -498 and -385, and a site in this region forms specific complexes in brain extract. A three-level model for lens-specific Pax6-dependent expression and gene recruitment is suggested: (i) binding of a specific isoform of Pax6; (ii) augmentation of expression through binding of Nrl or a related factor; and (iii) suppression of promoter activity in the central nervous system by an upstream negative element in the brain but not in the lens.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-1370456, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-1639073, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-1641336, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-1684639, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-1684738, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-1687460, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-1695712, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-1729696, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-1956382, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-1990971, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-212715, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-2178867, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-3013424, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-3349904, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-3569698, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-3595182, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-3904002, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-6235151, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-6306768, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-6960240, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-7476124, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-7482776, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-7590241, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-7628452, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-7753863, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-7817868, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-7867071, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-7890177, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-7891713, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-7892602, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-7914031, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-7917011, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-7935473, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-7951315, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-7958875, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-7979246, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-7984154, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-8107826, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-8108109, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-8114084, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-8125186, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-8132558, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-8170370, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-8236445, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-8246948, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-8264639, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-8288222, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-8552394, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-8552399, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-8552602, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-8663049, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-8674125, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-8760335, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-8787739, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-8787744, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-8787745, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-8827711, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-8939891, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-9037076, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-9163426, http://linkedlifedata.com/resource/pubmed/commentcorrection/9528779-9367909
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Basic-Leucine Zipper Transcription..., http://linkedlifedata.com/resource/pubmed/chemical/Crystallins, http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Eye Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Maf Transcription Factors, http://linkedlifedata.com/resource/pubmed/chemical/Nrl protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/PAX6 protein, http://linkedlifedata.com/resource/pubmed/chemical/Paired Box Transcription Factors, http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0270-7306
pubmed:author
pubmed:issnType
Print
pubmed:volume
18
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