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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1998-5-13
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pubmed:abstractText |
Expression of transgenes within a single generation by direct DNA injection into vertebrate embryos has been plagued by inefficient and nonuniform gene expression. We report a novel strategy for efficient and stable expression of transgenes driven by both ubiquitous and tissue-specific promoters by direct DNA injection into developing Xenopus laevis embryos. This strategy involves flanking expression cassettes of interest with inverted terminal repeat sequences (ITRs) from adeno-associated virus. Our results suggest that the ITR strategy may be generally applicable to other systems, such as zebra fish and embryonic stem cells, and may enable tissue-specific expression of transgenes in problematic contexts.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
|
|
pubmed:citationSubset |
IM
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pubmed:chemical |
|
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1087-0156
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
253-7
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:9528004-Actins,
pubmed-meshheading:9528004-Animals,
pubmed-meshheading:9528004-Animals, Genetically Modified,
pubmed-meshheading:9528004-Dependovirus,
pubmed-meshheading:9528004-Embryo, Nonmammalian,
pubmed-meshheading:9528004-Gene Expression Regulation, Developmental,
pubmed-meshheading:9528004-Genetic Engineering,
pubmed-meshheading:9528004-Genetic Vectors,
pubmed-meshheading:9528004-Green Fluorescent Proteins,
pubmed-meshheading:9528004-Heart,
pubmed-meshheading:9528004-Luminescent Proteins,
pubmed-meshheading:9528004-Organ Specificity,
pubmed-meshheading:9528004-Plasmids,
pubmed-meshheading:9528004-Promoter Regions, Genetic,
pubmed-meshheading:9528004-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:9528004-Somites,
pubmed-meshheading:9528004-Transgenes,
pubmed-meshheading:9528004-Xenopus laevis,
pubmed-meshheading:9528004-beta-Galactosidase
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pubmed:year |
1998
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pubmed:articleTitle |
Viral sequences enable efficient and tissue-specific expression of transgenes in Xenopus.
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pubmed:affiliation |
Department of Medicine, University of California, San Diego, La Jolla 92093-0613, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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