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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1998-4-28
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pubmed:abstractText |
A relatively simple assay procedure for measuring the reactions catalyzed by polyuronic acid C-5 epimerases has been developed. Action of C-5 epimerases inverts the C-6 carboxyl group of polyuronic acids converting beta-linked residues into alpha-linked residues or vice versa. The assay takes advantage of the greater susceptibility of the acid hydrolysis of alpha-glycosidic linkages than beta-glycosidic linkages. The method involves the partial acid hydrolysis of the polyuronic acid before and after reaction with the C-5 epimerase. The greater or lesser amounts of uronic acid released (solubilized) before and after reaction of the C-5 epimerase are a measure of the amount of alpha- or beta-glycosidic linkages that are formed and a measure of the amount of catalysis by the enzyme.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0003-2697
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 1998 Academic Press.
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pubmed:issnType |
Print
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pubmed:day |
10
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pubmed:volume |
258
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
59-62
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9527848-Alginates,
pubmed-meshheading:9527848-Azotobacter vinelandii,
pubmed-meshheading:9527848-Carbohydrate Conformation,
pubmed-meshheading:9527848-Carbohydrate Epimerases,
pubmed-meshheading:9527848-Glucuronates,
pubmed-meshheading:9527848-Glucuronic Acid,
pubmed-meshheading:9527848-Hexuronic Acids,
pubmed-meshheading:9527848-Hydrogen-Ion Concentration,
pubmed-meshheading:9527848-Hydrolysis,
pubmed-meshheading:9527848-Kinetics,
pubmed-meshheading:9527848-Spectrophotometry,
pubmed-meshheading:9527848-Uronic Acids
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pubmed:year |
1998
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pubmed:articleTitle |
Measurement of the activity of polyuronic acid C-5 epimerases.
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pubmed:affiliation |
Laboratory of Carbohydrate Chemistry and Enzymology, Iowa State University, Ames, Iowa 50011, USA.
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pubmed:publicationType |
Journal Article
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