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rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
3
|
|
pubmed:dateCreated |
1998-5-20
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|
pubmed:abstractText |
The introduction of several mutations resulted in the generation of improved mutants of the green fluorescent protein (GFP). A strong green (GFPsg25) and blue (BFPsg50) fluorescent protein, gave 50-fold-100-fold brighter fluorescence compared to wild-type GFP and BFP (Tyr66His), respectively, upon expression in mammalian cells. GFPsg25 and BFPsg50 have different excitation and emission maxima. This allows their use as an efficient dual-color tagging system and their independent detection in living cells.
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pubmed:language |
eng
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|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Mar
|
|
pubmed:issn |
0736-6205
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
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|
pubmed:volume |
24
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
462-6, 468-71
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|
pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9526659-3T3 Cells,
pubmed-meshheading:9526659-Amino Acid Substitution,
pubmed-meshheading:9526659-Animals,
pubmed-meshheading:9526659-Biological Markers,
pubmed-meshheading:9526659-Flow Cytometry,
pubmed-meshheading:9526659-Gene Expression Regulation,
pubmed-meshheading:9526659-Genes, Reporter,
pubmed-meshheading:9526659-Green Fluorescent Proteins,
pubmed-meshheading:9526659-Luminescent Proteins,
pubmed-meshheading:9526659-Mice,
pubmed-meshheading:9526659-Mutation,
pubmed-meshheading:9526659-Recombinant Fusion Proteins,
pubmed-meshheading:9526659-Spectrometry, Fluorescence,
pubmed-meshheading:9526659-Transfection
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|
pubmed:year |
1998
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|
pubmed:articleTitle |
Development and applications of enhanced green fluorescent protein mutants.
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|
pubmed:affiliation |
National Cancer Institute-FCRDC, Frederick, MD, USA.
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|
pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|
