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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
|
pubmed:dateCreated |
1998-5-20
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pubmed:abstractText |
A method is described that allows simultaneous measurement of two spectrally distinguishable green fluorescent protein (GFP) mutants with a confocal microscope. In contrast to previously described methods, neither UV excitation nor repetition of scans is required. Therefore the method is well-suited to the long-time observation of living cells in three-dimensional microscopy and time series recording, as demonstrated with GFP-expressing Dictyostelium discoideum cells.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Mar
|
pubmed:issn |
0736-6205
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
24
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
|
pubmed:pagination |
458-61
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9526658-Animals,
pubmed-meshheading:9526658-Argon,
pubmed-meshheading:9526658-Cell Line, Transformed,
pubmed-meshheading:9526658-Dictyostelium,
pubmed-meshheading:9526658-Green Fluorescent Proteins,
pubmed-meshheading:9526658-Lasers,
pubmed-meshheading:9526658-Luminescent Proteins,
pubmed-meshheading:9526658-Microscopy, Confocal,
pubmed-meshheading:9526658-Mutation
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pubmed:year |
1998
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pubmed:articleTitle |
Simultaneous detection of two GFP spectral mutants during in vivo confocal microscopy of migrating Dictyostelium cells.
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pubmed:affiliation |
Universität München, Germany.
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pubmed:publicationType |
Research Support, Non-U.S. Gov't,
Technical Report
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