Linked Life Data

9526615

Source:http://linkedlifedata.com/resource/pubmed/id/9526615

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1998-4-29
pubmed:abstractText
PAG1/MEST is an imprinted gene mapping to human chromosome 7q32. In human embryos and placenta, PEG1 is expressed from the paternally derived allele only, while maternal and paternal alleles are transcribed in adult blood lymphocytes. We aimed at investigating the origin of the maternal PEG1 transcript by studying PEG1 mRNA in two maternal uniparental disomy 7 patients suffering from severe pre- and post-natal growth restriction. PEG1 expression has been characterised by RT-PCR from leukocytes RNA using several primer pairs. The distal coding region in PEG1 mRNA could be repeatedly amplified from mUPD patients blood cells, but no amplification could be performed from the first exon, suggesting that the first exon was not a component of the maternal PEG1 transcript. As six independent database sequences showed that exon 2 was exactly joined to a novel sequence unrelated to exon 1 but identical in the common region of the six sequences, we hypothesized that our observation of PEG1 expression in the two maternal uniparental disomy 7 patients could be explained by transcription of the maternal allele from an alternate maternal upstream promoter.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:issn
0003-3995
pubmed:author
pubmed:issnType
Print
pubmed:volume
40
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
211-5
pubmed:dateRevised
2004-11-17
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
PEG1 expression in maternal uniparental disomy 7.
pubmed:affiliation
Laboratoire de Biochimie et Génétique Moléculaire, Centre Hospitalier Cochin-Port-Royal, Paris, France.
pubmed:publicationType
Journal Article