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PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1998-4-21
pubmed:abstractText
We report the molecular characterization of rotavirus genotype P[8]G5 strains found in fecal specimens collected in four different regions of Brazil, using digoxigenin(dig)-labeled oligonucleotide probes, sequence analysis, and RNA-RNA hybridization. The closest sequence relationships of the neutralization antigens of these strains were to the VP4 protein of P1A[8]G1 strain KU (93.3% identity in amino acids 11 to 282) and to the VP7 protein of G serotype 5 strain OSU (87.6% identity in amino acids 8 to 232). Based on VP7 sequence differences, we designed dig-probes that allowed us to discriminate porcine OSU-like strains from G5 strains isolated from Brazilian infants. The genetic relationships of two P[8]G5 isolates to other rotavirus genogroups were analyzed by RNA-RNA hybridization with [32P]-GTP probes representative of serotypes P1A[8]G1 (Wa), P[8]G3 (AU17), and P9[7]G5 (OSU). The Brazilian P[8]G5 strains showed sequence homology with genes of Wa-like and OSU-like strains, suggesting that these two strains were naturally occurring reassortants between members of the Wa and porcine rotavirus genogroups. The identification of these strains in diverse geographic areas of Brazil underscores their stability and demonstrates the emergence of clinically important rotavirus diarrhea strains by reassortment.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0304-8608
pubmed:author
pubmed:issnType
Print
pubmed:volume
141
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2353-64
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:9526542-Amino Acid Sequence, pubmed-meshheading:9526542-Antigens, Viral, pubmed-meshheading:9526542-Brazil, pubmed-meshheading:9526542-Capsid, pubmed-meshheading:9526542-Capsid Proteins, pubmed-meshheading:9526542-Cells, Cultured, pubmed-meshheading:9526542-Child, Preschool, pubmed-meshheading:9526542-Feces, pubmed-meshheading:9526542-Genes, Viral, pubmed-meshheading:9526542-Humans, pubmed-meshheading:9526542-Infant, pubmed-meshheading:9526542-Infant, Newborn, pubmed-meshheading:9526542-Molecular Epidemiology, pubmed-meshheading:9526542-Molecular Sequence Data, pubmed-meshheading:9526542-Nucleic Acid Hybridization, pubmed-meshheading:9526542-Polymerase Chain Reaction, pubmed-meshheading:9526542-RNA, Viral, pubmed-meshheading:9526542-Rotavirus, pubmed-meshheading:9526542-Rotavirus Infections, pubmed-meshheading:9526542-Sequence Alignment, pubmed-meshheading:9526542-Sequence Analysis, RNA, pubmed-meshheading:9526542-Sequence Homology, Amino Acid
pubmed:year
1996
pubmed:articleTitle
Characterization of human rotavirus genotype P[8]G5 from Brazil by probe-hybridization and sequence.
pubmed:affiliation
Laboratory of Animal Virology, Londrina State University, Brazil.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't