Linked Life Data

9526006

Source:http://linkedlifedata.com/resource/pubmed/id/9526006

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1998-4-20
pubmed:abstractText
Retinal ganglion cells (RGC) are supported by brain-derived neurotrophic factor (BDNF), but it is not known if BDNF acts as a target-derived factor or as an afferent or autocrine trophic factor. Here we demonstrate that BDNF mRNA is expressed in the retinorecipient layer of the chick optic tectum as well as in the inner nuclear layer and ganglion cell layer of the retina. Amacrine cells rather than RGC were the main source of BDNF mRNA in the ganglion cell layer, as determined by in situ hybridization that was combined with retrograde labeling of RGC and destruction of RGC by optic stalk transection, followed by quantitative RT-PCR. Cells in the ganglion cell layer as well as the retinorecipient layers of the optic tectum were BDNF-immunolabeled. After injections into the tectum, radio-iodinated BDNF was transported to the retina where autoradiographic label accumulated in the inner plexiform and ganglion cell layers. After intraocular injection, iodinated BDNF accumulated in these same retinal layers and correlated with the distribution of p75 neurotrophin receptor protein. The majority of cross-linked receptor-bound BDNF in the retina immunoprecipitated with p75 antibodies. No difference in the intensity of BDNF immunolabel was observed in the experimental retina or tectum after optic stalk transection, indicating that most of the BDNF in the RGC was not derived from the optic tectum. These data indicate that a substantial fraction of the BDNF in the ganglion cell layer is derived from local sources, afferents within the retina, rather than from the optic tectum via retrograde transport.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0270-6474
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2891-906
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:9526006-Animals, pubmed-meshheading:9526006-Axonal Transport, pubmed-meshheading:9526006-Brain-Derived Neurotrophic Factor, pubmed-meshheading:9526006-Chick Embryo, pubmed-meshheading:9526006-Cross-Linking Reagents, pubmed-meshheading:9526006-Gene Expression Regulation, Developmental, pubmed-meshheading:9526006-Immunohistochemistry, pubmed-meshheading:9526006-In Situ Hybridization, pubmed-meshheading:9526006-Microinjections, pubmed-meshheading:9526006-Neuroprotective Agents, pubmed-meshheading:9526006-Paracrine Communication, pubmed-meshheading:9526006-Precipitin Tests, pubmed-meshheading:9526006-RNA, Messenger, pubmed-meshheading:9526006-Receptor, Ciliary Neurotrophic Factor, pubmed-meshheading:9526006-Receptor, Nerve Growth Factor, pubmed-meshheading:9526006-Receptor Protein-Tyrosine Kinases, pubmed-meshheading:9526006-Receptors, Nerve Growth Factor, pubmed-meshheading:9526006-Retinal Ganglion Cells, pubmed-meshheading:9526006-Superior Colliculi
pubmed:year
1998
pubmed:articleTitle
Contributions of the optic tectum and the retina as sources of brain-derived neurotrophic factor for retinal ganglion cells in the chick embryo.
pubmed:affiliation
Department of Neurobiochemistry, Max-Planck-Institut for Psychiatry, D-82152 Martinsried, Germany.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't