Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1998-4-29
pubmed:abstractText
Starburst dendrimer, a structurally defined, spherical macromolecule composed of repeating polyamidoamino subunits, was investigated to augment plasmid-mediated gene transfer efficiency in a murine cardiac transplantation model. The grafts were directly injected with naked pCH110, a plasmid encoding beta-galactosidase (beta-Gal), or pCH110-dendrimer complex, and reporter gene expression determined by X-Gal staining. The grafts injected with pCH110-dendrimer demonstrated widespread and extended beta-Gal expression in both myocytes and the graft infiltrating cells from 7 to 28 days, compared to the grafts injected with naked pCH110 that expressed beta-Gal only in myocytes for less than 14 days. p alphaMHC-vIL-10, as plasmid encoding viral interleukin-10 (vIL-10) under the control of alpha-myosin heavy chain promoter, was able to prolong allograft survival from 13.9 +/- 0.9 days to 21.4 +/- 2.3 days (p < 0.005). When dendrimer G5EDA was used with p alphaMHC-vIL-10, 60-fold less DNA resulted in significant prolongation of graft survival to 38.6 +/- 4.7 days (p < 0.0005). The dose of DNA, the charge ratio of DNA to dendrimer, and the size generation of the dendrimers were all determined to be critical variables for prolongation of allograft survival in this model system. Thus, the use of the Starburst dendrimer dramatically increased the efficiency of plasmid-mediated gene transfer and expression. Production of immunosuppressive cytokines at higher amounts for longer periods of time in a greater expanse of tissue enhanced the immunosuppressive effect and prolonged graft survival further.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1043-0342
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
9
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
553-60
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:9525316-Animals, pubmed-meshheading:9525316-Dendrimers, pubmed-meshheading:9525316-Ear, External, pubmed-meshheading:9525316-Gene Expression, pubmed-meshheading:9525316-Gene Transfer Techniques, pubmed-meshheading:9525316-Genetic Vectors, pubmed-meshheading:9525316-Graft Survival, pubmed-meshheading:9525316-Heart Transplantation, pubmed-meshheading:9525316-Immunosuppressive Agents, pubmed-meshheading:9525316-Interleukin-10, pubmed-meshheading:9525316-Mice, pubmed-meshheading:9525316-Mice, Inbred Strains, pubmed-meshheading:9525316-Myocardium, pubmed-meshheading:9525316-Plasmids, pubmed-meshheading:9525316-Polyamines, pubmed-meshheading:9525316-Recombinant Fusion Proteins, pubmed-meshheading:9525316-Transplantation, Heterotopic, pubmed-meshheading:9525316-beta-Galactosidase
pubmed:year
1998
pubmed:articleTitle
Efficient transfer of genes into murine cardiac grafts by Starburst polyamidoamine dendrimers.
pubmed:affiliation
Department of Surgery and Microbiology and Immunology, University of Michigan, Ann Arbor 48109-0331, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't