Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1998-5-4
pubmed:abstractText
Hepatitis C virus (HCV) often causes a prolonged and persistent infection, and an association between hepatocellular carcinoma (HCC) and HCV infection has been noted. Recent experimental evidence using a cloned genomic region suggests that the putative core protein of HCV has numerous biological properties and is implicated as a viral factor for HCV mediated pathogenesis. WAF1/Cip1/Sid1 (p21) is the prototype of a family of proteins that inhibit cyclin-dependent kinases (CDK) and regulate cell cycle progression in eukaryotic cells. In this study, we have observed that the HCV core protein represses the transcriptional activity of the p21 promoter when tested separately by an in-vitro transient expression assay using murine fibroblasts (NIH3T3), human hepatocellular carcinoma (HepG2), and human cervical carcinoma (HeLa) cells. A deletion analysis of the p21 promoter suggested that the HCV core responsive region is located downstream of the p53 binding site. A gel mobility shift analysis showed that the HCV core protein does not bind directly to p21 regulatory sequences. Thus, the HCV core protein appears to act as an effector in the promotion of cell growth by repressing p21 transcription through unknown cellular factor(s).
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:day
27
pubmed:volume
208
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
331-6
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:9524287-Animals, pubmed-meshheading:9524287-Carcinoma, Hepatocellular, pubmed-meshheading:9524287-Cell Line, pubmed-meshheading:9524287-Cyclin-Dependent Kinase Inhibitor p21, pubmed-meshheading:9524287-Cyclins, pubmed-meshheading:9524287-Glutathione Transferase, pubmed-meshheading:9524287-HeLa Cells, pubmed-meshheading:9524287-Hepacivirus, pubmed-meshheading:9524287-Hepatitis C Antigens, pubmed-meshheading:9524287-Humans, pubmed-meshheading:9524287-Liver Neoplasms, pubmed-meshheading:9524287-Luciferases, pubmed-meshheading:9524287-Mice, pubmed-meshheading:9524287-Promoter Regions, Genetic, pubmed-meshheading:9524287-Recombinant Fusion Proteins, pubmed-meshheading:9524287-Transfection, pubmed-meshheading:9524287-Tumor Cells, Cultured, pubmed-meshheading:9524287-Viral Core Proteins
pubmed:year
1998
pubmed:articleTitle
Hepatitis C virus core protein represses p21WAF1/Cip1/Sid1 promoter activity.
pubmed:affiliation
Division of Infectious Diseases and Immunology, Saint Louis University, MO 63110, USA. rayrb@sluvca.slu.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.