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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
1998-5-7
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pubmed:databankReference |
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pubmed:abstractText |
Feline Interleukin-12 (IL-12) is a heterodimeric glycoprotein consisting of two disulfide linked subunits of about 40 kD (p40) and 35 kD (p35). It is a pleiotropic cytokine mediating biological activities on T- and NK-cells. One important function is the induction of a Th1 immune response. Here we report the cloning and sequencing of feline IL-12, the expression of the p40-protein in E. coli and production of monoclonal antibodies. At the nucleotide level, feline IL-12 shows between 87-90%, on the amino acid level between 82-87% identity to the bovine and human IL-12, respectively.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
1042-5179
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
8
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
77-82
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9522125-Amino Acid Sequence,
pubmed-meshheading:9522125-Animals,
pubmed-meshheading:9522125-Antibodies, Monoclonal,
pubmed-meshheading:9522125-Base Sequence,
pubmed-meshheading:9522125-Cats,
pubmed-meshheading:9522125-Cattle,
pubmed-meshheading:9522125-Cloning, Molecular,
pubmed-meshheading:9522125-Cytoplasm,
pubmed-meshheading:9522125-Escherichia coli,
pubmed-meshheading:9522125-Glycosylation,
pubmed-meshheading:9522125-Humans,
pubmed-meshheading:9522125-Interleukin-12,
pubmed-meshheading:9522125-Mice,
pubmed-meshheading:9522125-Mice, Inbred BALB C,
pubmed-meshheading:9522125-Molecular Sequence Data,
pubmed-meshheading:9522125-Rabbits,
pubmed-meshheading:9522125-Recombinant Proteins,
pubmed-meshheading:9522125-Sequence Analysis,
pubmed-meshheading:9522125-Sequence Homology, Amino Acid
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pubmed:year |
1997
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pubmed:articleTitle |
Nucleotide and predicted peptide sequence of feline interleukin-12 (IL-12).
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pubmed:affiliation |
Dept. of Internal Veterinary Medicine, University of Zurich, Switzerland. danifehr@vetklinik.unizh.ch
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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