Linked Life Data

9520108

Source:http://linkedlifedata.com/resource/pubmed/id/9520108

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1998-4-29
pubmed:abstractText
We developed a new method to estimate the magnitude of differential cell-cell adhesion of two tissues based on the spatial patterns in cell-sorting experiments, and applied it to experimental data on progress-zone cells of avian limb bud at stages 20-26. The change in cell distribution in the experiment was recorded, and statistics qB/B for the degree of cell sorting was calculated from the photographs. Based on extensive computer simulations of spatial Markov processes on a 2-D lattice, we derived a formula for qB/B increasing with time. Using least square fitting, differential adhesion A and cell motility m are estimated from the time series data of qB/B obtained from the experiment. The estimated A was close to 0 (the spatial pattern remained random) if the mixed cells were from two tissues of the same stage. If the mixed cells were from different stages, and the estimated A was positive (cell sorting occurred). Estimated A increased with the difference in the stage number of the two tissues from which the cells were sampled. This result can be explained both by the stage-specific change in adhesion molecules and by a linear increase (or decrease) in the amount of adhesion molecules on cell surface.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1058-8388
pubmed:author
pubmed:issnType
Print
pubmed:volume
211
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
204-14
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Cell-cell adhesion in limb-formation, estimated from photographs of cell sorting experiments based on a spatial stochastic model.
pubmed:affiliation
Department of Biology, Faculty of Science, Kyushu University, Fukuoka, Japan. amochscb@mbox.nc.kyushu-u.ac.jp
pubmed:publicationType
Journal Article