9517478

pubmed:Citation

12

In the present study, we demonstrate that choline, a precursor of acetylcholine (ACh) and a product of acetylcholine hydrolysis by acetylcholinesterase (AChE), acts as an efficient and relatively selective agonist of alpha7-containing nicotinic acetylcholine receptors (nAChR) in neurons cultured from the rat hippocampus, olfactory bulb and thalamus as well as in PC12 cells. Choline was able to activate postsynaptic and presynaptic alpha7 nAChRs, with the latter action resulting in the release of other neurotransmitters. Although choline was approximately one order of magnitude less potent than ACh (EC50 of 1.6 mM for choline and 0.13 mM for ACh), it acted as a full agonist at alpha7 nAChRs. In contrast, choline did not activate alpha4beta2 agonist-bearing nAChRs on hippocampal neurons, and acted as a partial agonist at alpha3beta4-containing nAChRs on PC12 cells. The ethyl alcohol moiety of choline is required for the selective action on alpha7 nAChR. Exposure of cultured hippocampal neurons for 10 min to choline (10-100 microM) resulted in desensitization of the native alpha7 nAChRs. Moreover, chronic exposure (10 days) of the cultured hippocampal neurons to a desensitizing concentration of choline (approximately 30 microM) decreased their responsiveness to ACh. The selective action of choline on native alpha7 nAChRs suggests that this naturally occurring compound may act in vivo as an endogenous ligand for these receptors. Putative physiological actions of choline include retrograde messenger activity during the development of the mammalian central nervous system and during periods of elevated synaptic activity that leads to long-term potentiation.

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MEDLINE

0953-816X

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NLM

2734-42

pubmed-meshheading:9517478-Acetylcholine, pubmed-meshheading:9517478-Animals, pubmed-meshheading:9517478-Bungarotoxins, pubmed-meshheading:9517478-Choline, pubmed-meshheading:9517478-Dimethylphenylpiperazinium Iodide, pubmed-meshheading:9517478-Excitatory Amino Acid Agonists, pubmed-meshheading:9517478-Hippocampus, pubmed-meshheading:9517478-Mecamylamine, pubmed-meshheading:9517478-Methylamines, pubmed-meshheading:9517478-N-Methylaspartate, pubmed-meshheading:9517478-Neurons, pubmed-meshheading:9517478-Nicotinic Agonists, pubmed-meshheading:9517478-Nicotinic Antagonists, pubmed-meshheading:9517478-Nootropic Agents, pubmed-meshheading:9517478-Olfactory Bulb, pubmed-meshheading:9517478-PC12 Cells, pubmed-meshheading:9517478-Patch-Clamp Techniques, pubmed-meshheading:9517478-Rats, pubmed-meshheading:9517478-Rats, Sprague-Dawley, pubmed-meshheading:9517478-Thalamus

Choline is a selective agonist of alpha7 nicotinic acetylcholine receptors in the rat brain neurons.

Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't