Linked Life Data

9517462

Source:http://linkedlifedata.com/resource/pubmed/id/9517462

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1998-4-24
pubmed:abstractText
The transient expression of neuropeptide transmitters is a common feature of the developing cortex. We have now analysed the role of cortical afferents in shaping the neurochemical architecture of rat visual cortex using organotypic cultures. Deafferented cortex monocultures prepared from newborn rats reveal a constant NPY mRNA expression in 6-8% of all cortical neurons up to 90 days in vitro (DIV). In contrast, afferent thalamocortical and corticocortical axonal innervation elicits a progressive reduction in the percentage of NPY mRNA expressing neurons from initially 6-8% in 30DIV cocultures to 2-3% and 3-4% respectively in 60DIV cocultures, which is maintained for up to 90DIV. This phenotype restriction is not observed in only efferently connected corticocollicular cocultures. Further, axonal innervation does not change the percentage of GAD mRNA-expressing neurons, which remains at 13% in mono- and cocultures. When feeding thalamocortical cocultures with monoculture-conditioned medium between 3-20DIV followed by normal medium up to 60DIV, the phenotype restriction fails to occur in the cocultured cortex. We conclude that cortex-derived factors secreted into the medium by a monoculture suppress the phenotype-restricting capacity of the afferents, but only when present within the first 14DIV during the period of formation of axonal connections. To elucidate the nature of the cortex-derived factors, brain-derived neurotrophic factor was applied to the medium. When applied for the first 14DIV, it does not prevent the phenotype restriction from occurring. This suggests that epigenetic factors such as axonal innervation and cortex-derived factors other than brain-derived neurotrophic factor govern a phenotype decision in neocortical neurons during a period of molecular plasticity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0953-816X
pubmed:author
pubmed:issnType
Print
pubmed:volume
9
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2571-80
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Phenotype specification of cortical neurons during a period of molecular plasticity.
pubmed:affiliation
AG Entwicklungsneurobiologie, Fakultät für Biologie, Ruhr-Universität, Bochum, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't