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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
13
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pubmed:dateCreated |
1998-4-23
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pubmed:abstractText |
Grb2 is an adaptor molecule comprising one Src homology (SH) 2 and two SH3 domains. This protein has a natural isoform named Grb3-3 with a deletion within the SH2 domain. Numerous evidence points to a functional connection between SH2- and SH3-containing proteins and molecules implicated in RNA biogenesis. In this context, we have examined the binding of Grb2 and Grb3-3 to heterogeneous nuclear ribonucleoprotein (hnRNP) C. By the use of an in vivo genetic approach and through in vitro experiments, we furnish evidence that both Grb2 and Grb3-3 interact with hnRNP C proteins. Subcellular fractionation studies clearly show that Grb2 is partially localized in the nucleus. In addition, coimmunoprecipitation experiments demonstrate that Grb2.hnRNP C complexes exist in intact hematopoietic cells. The carboxyl-terminal SH3 domains of Grb2 and Grb3-3 are primarily responsible for the association with hnRNP C. However, although the proline-rich motif of hnRNP C is involved in the interaction with Grb2, it is not in the binding to Grb3-3. Furthermore, poly(U) RNA inhibits the association of Grb2 with hnRNP C, whereas it enhances the interaction between Grb3-3 and hnRNP C. These findings suggest that the Grb2/Grb3-3-hnRNP C interactions might fulfill different biological functions.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adaptor Proteins, Signal Transducing,
http://linkedlifedata.com/resource/pubmed/chemical/GRB2 Adaptor Protein,
http://linkedlifedata.com/resource/pubmed/chemical/GRB2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Grb2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Heterogeneous-Nuclear...,
http://linkedlifedata.com/resource/pubmed/chemical/Heterogeneous-Nuclear...,
http://linkedlifedata.com/resource/pubmed/chemical/Poly U,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Heterogeneous Nuclear,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Epidermal Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Ribonucleoproteins
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
27
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pubmed:volume |
273
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
7776-81
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:9516488-3T3 Cells,
pubmed-meshheading:9516488-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:9516488-Animals,
pubmed-meshheading:9516488-Apoptosis,
pubmed-meshheading:9516488-Cloning, Molecular,
pubmed-meshheading:9516488-GRB2 Adaptor Protein,
pubmed-meshheading:9516488-Heterogeneous-Nuclear Ribonucleoprotein Group C,
pubmed-meshheading:9516488-Heterogeneous-Nuclear Ribonucleoproteins,
pubmed-meshheading:9516488-Humans,
pubmed-meshheading:9516488-Jurkat Cells,
pubmed-meshheading:9516488-Mice,
pubmed-meshheading:9516488-Mutagenesis, Site-Directed,
pubmed-meshheading:9516488-Poly U,
pubmed-meshheading:9516488-Proteins,
pubmed-meshheading:9516488-RNA, Heterogeneous Nuclear,
pubmed-meshheading:9516488-Receptor, Epidermal Growth Factor,
pubmed-meshheading:9516488-Ribonucleoproteins,
pubmed-meshheading:9516488-Saccharomyces cerevisiae,
pubmed-meshheading:9516488-src Homology Domains
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pubmed:year |
1998
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pubmed:articleTitle |
Grb2 and its apoptotic isoform Grb3-3 associate with heterogeneous nuclear ribonucleoprotein C, and these interactions are modulated by poly(U) RNA.
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pubmed:affiliation |
Institut Cochin de Génétique Moléculaire, U363 INSERM, Hôpital Cochin, 27 rue du faubourg Saint Jacques, 75014 Paris, France. romero@icgm.cochin.inserm.fr
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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