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Predicate | Object |
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rdf:type | |
lifeskim:mentions |
umls-concept:C0001613,
umls-concept:C0007776,
umls-concept:C0008546,
umls-concept:C0012854,
umls-concept:C0022655,
umls-concept:C0025914,
umls-concept:C0026809,
umls-concept:C0027540,
umls-concept:C0027882,
umls-concept:C0205182,
umls-concept:C0220839,
umls-concept:C0233656,
umls-concept:C0235032,
umls-concept:C0336762,
umls-concept:C0598405
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pubmed:issue |
3
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pubmed:dateCreated |
1998-4-29
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pubmed:abstractText |
The possibility that glutamate may induce neuronal apoptosis was examined in cultured cortical neurons. Neurons underwent widespread death 24 h following exposure to 50 microM glutamate. The glutamate neurotoxicity was blocked by inclusion of the glutamate antagonists, 10 microM MK-801 and 50 microM CNQX. The death was characterized by swelling cell body and bursting cytoplasmic membrane in the early phase of degeneration, suggesting that glutamate produces receptor-mediated excitotoxic necrosis. With blockade of excitotoxicity by addition of 10 microM MK-801 and 50 microM CNQX, cortical neurons exposed to 2 mM glutamate underwent necrosis morphologically identical to excitotoxicity but sensitive to 100 microM trolox, an antioxidant, suggesting that high doses of glutamate produce oxidative neuronal necrosis via non-receptor-mediated mechanisms. Interestingly, internucleosomal DNA fragmentation and chromatin condensation were observed in the course of glutamate-induced neuronal necrosis.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0304-3940
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
16
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pubmed:volume |
240
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
147-50
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9502225-Animals,
pubmed-meshheading:9502225-Apoptosis,
pubmed-meshheading:9502225-Cells, Cultured,
pubmed-meshheading:9502225-Cerebral Cortex,
pubmed-meshheading:9502225-Chromatin,
pubmed-meshheading:9502225-DNA Fragmentation,
pubmed-meshheading:9502225-Fetus,
pubmed-meshheading:9502225-Glutamic Acid,
pubmed-meshheading:9502225-Mice,
pubmed-meshheading:9502225-Microscopy, Electron,
pubmed-meshheading:9502225-Necrosis
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pubmed:year |
1998
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pubmed:articleTitle |
Glutamate neurotoxicity in mouse cortical neurons: atypical necrosis with DNA ladders and chromatin condensation.
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pubmed:affiliation |
Laboratory of Cell Biology, Institute for Medical Sciences, Kyungkido, South Korea.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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