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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1998-4-10
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pubmed:abstractText |
Bacterial virulence factors are typically surface-associated or secreted molecules that in Gram-negative bacteria must cross the outer membrane (OM). Protein translocation across the bacterial OM is not well understood. To elucidate this process we studied P pilus biogenesis in Escherichia coli. We present high-resolution electron micrographs of the OM usher PapC and show that it forms an oligomeric complex containing a channel approximately 2 nm in diameter. This is large enough to accommodate pilus subunits or the linear tip fibrillum of the pilus but not large enough to accommodate the final 6.8-nm-wide helical pilus rod. We show that P pilus rods can be unraveled into linear fibers by incubation in 50% glycerol. Thus, they are likely to pass through the usher in this unwound form. Packaging of these fibers into their final helical structure would only occur outside the cell, a process that may drive outward growth of the pilus organelles. The usher complex appears to be similar to complexes formed by members of the PulD/pIV family of OM proteins, and thus these two protein families, previously thought to be unrelated, may share structural and functional homologies.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9501230-1348107,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9501230-1352770,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9501230-1361168,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9501230-1361583,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9501230-1370823,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9501230-1380671,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9501230-1683704,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9501230-1702438,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9501230-1812789,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9501230-1970114,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9501230-2585121,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9501230-2862689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9501230-2897064,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9501230-6296139,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9501230-6310323,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9501230-6769802,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9501230-7476178,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9501230-7648030,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9501230-7816100,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9501230-7901733,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9501230-7906265,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9501230-7909802,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9501230-8036510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9501230-8057896,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9501230-8096174,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9501230-8096622,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9501230-8097321,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/9501230-8302219,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9501230-8438237,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9501230-8576244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9501230-8577257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9501230-8605893,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9501230-8606171,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9501230-8670884,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9501230-8825101,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9501230-8830709,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9501230-8917515,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9501230-9141189,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9501230-9351822,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9501230-9374466,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9501230-9427408,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9501230-9466268
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
17
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pubmed:volume |
95
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3146-51
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9501230-Bacterial Outer Membrane Proteins,
pubmed-meshheading:9501230-Biological Transport,
pubmed-meshheading:9501230-Cell Membrane,
pubmed-meshheading:9501230-DNA Nucleotidyltransferases,
pubmed-meshheading:9501230-Escherichia coli,
pubmed-meshheading:9501230-Escherichia coli Proteins,
pubmed-meshheading:9501230-Fimbriae Proteins,
pubmed-meshheading:9501230-Integrases,
pubmed-meshheading:9501230-Porins,
pubmed-meshheading:9501230-Protein Conformation,
pubmed-meshheading:9501230-Protein Denaturation,
pubmed-meshheading:9501230-Protein Folding,
pubmed-meshheading:9501230-Recombinases
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pubmed:year |
1998
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pubmed:articleTitle |
The PapC usher forms an oligomeric channel: implications for pilus biogenesis across the outer membrane.
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pubmed:affiliation |
Department of Molecular Microbiology, Box 8230, 660 South Euclid Avenue, Washington University School of Medicine, St. Louis, MO 63110, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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