Linked Life Data

9498767

Source:http://linkedlifedata.com/resource/pubmed/id/9498767

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1998-3-18
pubmed:abstractText
Mouse bone marrow (BM) stromal cell conditioned medium (CM) from our long-term lymphoid culture system selectively induces the in vitro proliferation and presumptive differentiation of pre-pro-B cells (B220+, HSA-, TdT- or TdT+, c[mu-]) from adult rat, mouse, and human BM. However, the responsible growth factor(s) has not yet been identified. Inasmuch as IL-7 is one of the cytokines most closely associated with early B-lineage development, we utilized BM adherent cells and stromal cell lines from IL-7 gene-deleted (-/-) mice in combination with rIL-7 and anti-IL-7 mAb to investigate its possible regulatory role in our culture system. The results show that, although rIL-7 and IL-7 (-/-) CM each can maintain the viability of freshly harvested pre-pro-B cells in vitro, neither induces them to proliferate and/or differentiate, even in the presence of recombinant stem cell factor (rSCF) and/or recombinant insulin-like growth factor (rIGF). The results also show that anti-IL-7 mAb fails to neutralize the pre-pro-B cell growth-stimulating activity in IL-7 (+/+) CM. Yet rIL-7 enables IL-7 (-/-) CM to induce proliferation of pre-pro-B cells, and to "prime" them to respond directly to monomeric IL-7. Furthermore, anti-IL-7 mAb adsorbs the pre-pro-B cell growth-stimulating activity from both IL-7 (+/+) CM and rIL-7-supplemented IL-7 (-/-) CM; but rIL-7 does not restore this activity. Lastly, both pre-pro-B cell growth-stimulatory activity and IL-7 are quantitatively recovered by ultrafiltration in the 50 to 100 kDa, rather than the 10 to 50 kDa, apparent molecular mass fraction. These results suggest that the pre-pro-B cell growth-stimulating activity in our culture system is the property of a self-associating complex of IL-7 and a second BM stromal cell-derived cofactor.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
160
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2272-9
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:9498767-Animals, pubmed-meshheading:9498767-Antibodies, Monoclonal, pubmed-meshheading:9498767-B-Lymphocytes, pubmed-meshheading:9498767-Bone Marrow Cells, pubmed-meshheading:9498767-Cell Differentiation, pubmed-meshheading:9498767-Cells, Cultured, pubmed-meshheading:9498767-Chemokine CXCL12, pubmed-meshheading:9498767-Chemokines, pubmed-meshheading:9498767-Chemokines, CXC, pubmed-meshheading:9498767-Culture Media, Conditioned, pubmed-meshheading:9498767-Gene Deletion, pubmed-meshheading:9498767-Growth Substances, pubmed-meshheading:9498767-Interleukin-7, pubmed-meshheading:9498767-Macromolecular Substances, pubmed-meshheading:9498767-Male, pubmed-meshheading:9498767-Mice, pubmed-meshheading:9498767-Mice, Inbred C57BL, pubmed-meshheading:9498767-Mice, Knockout, pubmed-meshheading:9498767-Molecular Weight, pubmed-meshheading:9498767-Rats, pubmed-meshheading:9498767-Rats, Inbred Lew, pubmed-meshheading:9498767-Recombinant Proteins, pubmed-meshheading:9498767-Stem Cells, pubmed-meshheading:9498767-Stromal Cells
pubmed:year
1998
pubmed:articleTitle
Identification of an IL-7-associated pre-pro-B cell growth-stimulating factor (PPBSF). I. Production of the non-IL-7 component by bone marrow stromal cells from IL-7 gene-deleted mice.
pubmed:affiliation
Department of Pathology, School of Medicine, University of Connecticut Health Center, Farmington 06030, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.