Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1998-3-18
|
| pubmed:abstractText |
Cutaneous painting with reactive haptens induces contact sensitivity (CS) responses that are in vivo examples of T cell immunity. In contrast, high dose i.v. administration of the hapten can induce tolerance. We investigated the effect of IL-12 on reversal of this tolerance and attempted to determine in vitro the mechanism of this reversing effect by measuring proliferation and IFN-gamma production by CS effector T cells stimulated with hapten-conjugated APC, and we also measured CS ear swelling in vivo. The in vitro responses of T cells to hapten-APC became absent in tolerized mice, paralleling impaired in vivo CS responses. Addition of IL-12 to cultures manifesting this fully established in vitro tolerance completely restored impaired responses of tolerized T cells. The reversing effects of IL-12 were not blocked by anti-IFN-gamma mAb, but were blocked by mAbs against B7-1, more strongly by anti-B7-2, and by both Abs together. Additional in vivo ear-swelling response experiments confirmed the reversing effects of IL-12 on established tolerance. To examine whether the IL-12 effect depended on stimulation of IFN-gamma, we directly injected IFN-gamma into tolerized mice. This partially mimicked but did not fully reconstitute the effects of IL-12. In summary, IL-12 abrogation of established tolerance of CS may have been partially due to endogenous production of IFN-gamma, but appeared mainly due to direct activation of the tolerized T cells by affecting signaling through costimulatory molecules B7-1 and B7-2.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD80,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD86,
http://linkedlifedata.com/resource/pubmed/chemical/Cd86 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Haptens,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-12,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
160
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2080-8
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:9498744-Animals,
pubmed-meshheading:9498744-Antigens, CD,
pubmed-meshheading:9498744-Antigens, CD80,
pubmed-meshheading:9498744-Antigens, CD86,
pubmed-meshheading:9498744-Cells, Cultured,
pubmed-meshheading:9498744-Dermatitis, Contact,
pubmed-meshheading:9498744-Edema,
pubmed-meshheading:9498744-Epitopes,
pubmed-meshheading:9498744-Haptens,
pubmed-meshheading:9498744-Immune Tolerance,
pubmed-meshheading:9498744-Injections, Intravenous,
pubmed-meshheading:9498744-Interferon-gamma,
pubmed-meshheading:9498744-Interleukin-12,
pubmed-meshheading:9498744-Lymph Nodes,
pubmed-meshheading:9498744-Lymphocyte Activation,
pubmed-meshheading:9498744-Male,
pubmed-meshheading:9498744-Membrane Glycoproteins,
pubmed-meshheading:9498744-Mice,
pubmed-meshheading:9498744-Mice, Inbred CBA,
pubmed-meshheading:9498744-T-Lymphocytes
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
IL-12 reverses established antigen-specific tolerance of contact sensitivity by affecting costimulatory molecules B7-1 (CD80) and B7-2 (CD86).
|
| pubmed:affiliation |
Department of Veterinary Surgery, University of Osaka Prefecture College of Agriculture, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|