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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1998-4-9
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pubmed:abstractText |
This study investigated the modulatory effect of magnesium (Mg++) on basal and agonist-stimulated intracellular free calcium (Ca++) concentration ([Ca++]i) in vascular smooth muscle cells from spontaneously hypertensive rats (SHR). Effects of increasing extracellular Mg++ concentration ([Mg++]e) on vasopressin (AVP)-induced [Ca++]i responses were determined in primary cultured unpassaged vascular smooth muscle cells from mesenteric and aortic vessels (representing resistance and conduit arteries, respectively) of Wistar Kyoto rats (WKY) and SHR. [Ca++]i was measured by fura-2 methodology. Underlying mechanisms for Mg++ actions were determined in Ca(++)-free buffer and in the presence of diltiazem (10(-6) M), an L-type Ca++ channel blocker. Basal and AVP-stimulated [Ca++]i responses were significantly increased (p < .05) in SHR (pD2 = 8.3 +/- 0.1, Emax = 532 +/- 14 nM for SHR; pD2 = 8.0 +/- 0.04, Emax = 480 +/- 15 nM for WKY). [Mg++]e dose-dependently reduced basal and agonist-induced [Ca++]i responses. High [Mg++]e (4.8 mM) attenuated [Ca++]i responses to AVP in WKY (Emax = 328 +/- 30 nM) and SHR (Emax = 265 +/- 27 nM) and normalized AVP-elicited hyper-responsiveness in SHR (pD2 in high [Mg++]e, 8.1 +/- 0.3 for SHR, 7.8 +/- 0.6 for WKY). Extracellular Ca++ withdrawal and diltiazem abolished the attenuating effects of high [Mg++]e in WKY but not in SHR. These findings demonstrate that Mg++ dose-dependently reduces [Ca++]i and that high [Mg++]e attenuates AVP-stimulated [Ca++]i responses and normalizes sensitivity to AVP in SHR. In WKY, Mg++ actions are dependent primarily on Ca++ influx through L-type Ca++ channels, whereas in SHR, the modulatory effects of [Mg++]e are mediated both by Ca++ influx through Ca++ channels and by intracellular Ca++ release.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0022-3565
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
284
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
998-1005
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9495860-Animals,
pubmed-meshheading:9495860-Arginine Vasopressin,
pubmed-meshheading:9495860-Calcium,
pubmed-meshheading:9495860-Cells, Cultured,
pubmed-meshheading:9495860-Dose-Response Relationship, Drug,
pubmed-meshheading:9495860-Hypertension,
pubmed-meshheading:9495860-Magnesium,
pubmed-meshheading:9495860-Male,
pubmed-meshheading:9495860-Muscle, Smooth, Vascular,
pubmed-meshheading:9495860-Rats,
pubmed-meshheading:9495860-Rats, Inbred SHR,
pubmed-meshheading:9495860-Rats, Inbred WKY
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pubmed:year |
1998
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pubmed:articleTitle |
Effect of magnesium on calcium responses to vasopressin in vascular smooth muscle cells of spontaneously hypertensive rats.
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pubmed:affiliation |
MRC Multidisciplinary Research Group on Hypertension, University of Montreal, Quebec, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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