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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
|
pubmed:dateCreated |
1976-10-1
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pubmed:abstractText |
A method for measuring the rate of protein degradation is described. The method measures the change in 2-3H content of protein with time by racemization of the protein hydrolysate with acetic anhydride. The 3H on C-2 of amino acids is stable in proteins but becomes labile, owing to the action of transaminases, once the amino acids are released by proteolysis. The specific measurement of 2-3H in amino acids largely overcomes problems due to compartmentation and isotope recycling and evidence to support this claim is presented. Values for the half-life of Lemna minor (duckweed) protein determined by the new method are compared with values obtained by other methods.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/949338-1156391,
http://linkedlifedata.com/resource/pubmed/commentcorrection/949338-16657385,
http://linkedlifedata.com/resource/pubmed/commentcorrection/949338-4209898,
http://linkedlifedata.com/resource/pubmed/commentcorrection/949338-5538614
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pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Jun
|
pubmed:issn |
0264-6021
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pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
15
|
pubmed:volume |
156
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
561-8
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pubmed:dateRevised |
2010-9-3
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pubmed:meshHeading | |
pubmed:year |
1976
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pubmed:articleTitle |
A sensitive method for measuring protein turnover based on the measurement of 2-3H-labelled amino acids in protein.
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pubmed:publicationType |
Journal Article
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