pubmed-article:949325 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:949325 | lifeskim:mentions | umls-concept:C0034493 | lld:lifeskim |
pubmed-article:949325 | lifeskim:mentions | umls-concept:C0004793 | lld:lifeskim |
pubmed-article:949325 | lifeskim:mentions | umls-concept:C0035696 | lld:lifeskim |
pubmed-article:949325 | lifeskim:mentions | umls-concept:C0017645 | lld:lifeskim |
pubmed-article:949325 | lifeskim:mentions | umls-concept:C0349590 | lld:lifeskim |
pubmed-article:949325 | lifeskim:mentions | umls-concept:C0444930 | lld:lifeskim |
pubmed-article:949325 | pubmed:issue | 3 | lld:pubmed |
pubmed-article:949325 | pubmed:dateCreated | 1976-10-1 | lld:pubmed |
pubmed-article:949325 | pubmed:abstractText | Poly(A)-containing messenger RNA isolated from rabbit reticulocytes as estimated by periodate oxidation and condensation with [3H]isoniazid has two oxidizable end groups per molecule of mol. wt. 220000. When the mRNA is subjected to stepwise degradation by beta-elimination, only one oxidizable end-group is found. This indicates that one of the 2',3' hydroxyl end-groups is linked through the normal 3'--5' phosphodiester bond, but that the other is linked in such a way that after stepwise degradation no new 2',3 hydroxyl group is revealed. This structure could be a 5'-linked 5'-phospho di- or tri-ester. On digestion with ribonuclease the isoniazid-labelled RNA produced oligonucleotide hydrazones consistent with a poly(A) sequence at the 3' end plus fragments that are not found after stepwise degradation. These fragments have a charge of --6 and --8 from pancreatic ribonuclease or --7 from ribonuclease T1 digestion. These charges are changed to --3.4 and --4.1 after pancreatic ribonuclease, ribonuclease T2 and alkaline phosphatase digestion. methyl-3H-labelled-poly(A)-containing RNA isolated from late erythroid cells contain a methyl-labelled fragment resistant to endonuclease and phosphodiesterase II digestion. After digestion with phosphodiesterase I this fragment produces methyl-3 H-labelled nucleotides with the electrophoretic mobility of pm7G and pAm. It is concluded that globin mRNA has the 5' sequences m7G(5')ppp'AmpYpGp ... and m7G(5')pppAmpApGpYp. | lld:pubmed |
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pubmed-article:949325 | pubmed:language | eng | lld:pubmed |
pubmed-article:949325 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:949325 | pubmed:citationSubset | IM | lld:pubmed |
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pubmed-article:949325 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:949325 | pubmed:month | Jun | lld:pubmed |
pubmed-article:949325 | pubmed:issn | 0264-6021 | lld:pubmed |
pubmed-article:949325 | pubmed:author | pubmed-author:HuntJ AJA | lld:pubmed |
pubmed-article:949325 | pubmed:author | pubmed-author:OakesG NGN | lld:pubmed |
pubmed-article:949325 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:949325 | pubmed:day | 1 | lld:pubmed |
pubmed-article:949325 | pubmed:volume | 155 | lld:pubmed |
pubmed-article:949325 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:949325 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:949325 | pubmed:pagination | 637-44 | lld:pubmed |
pubmed-article:949325 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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pubmed-article:949325 | pubmed:year | 1976 | lld:pubmed |
pubmed-article:949325 | pubmed:articleTitle | The nature of the 5'-linked 5' nucleotide sequence at the 5' end of rabbit globin messenger ribonucleic acid. | lld:pubmed |
pubmed-article:949325 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:949325 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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