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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1976-10-1
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pubmed:abstractText |
Poly(A)-containing messenger RNA isolated from rabbit reticulocytes as estimated by periodate oxidation and condensation with [3H]isoniazid has two oxidizable end groups per molecule of mol. wt. 220000. When the mRNA is subjected to stepwise degradation by beta-elimination, only one oxidizable end-group is found. This indicates that one of the 2',3' hydroxyl end-groups is linked through the normal 3'--5' phosphodiester bond, but that the other is linked in such a way that after stepwise degradation no new 2',3 hydroxyl group is revealed. This structure could be a 5'-linked 5'-phospho di- or tri-ester. On digestion with ribonuclease the isoniazid-labelled RNA produced oligonucleotide hydrazones consistent with a poly(A) sequence at the 3' end plus fragments that are not found after stepwise degradation. These fragments have a charge of --6 and --8 from pancreatic ribonuclease or --7 from ribonuclease T1 digestion. These charges are changed to --3.4 and --4.1 after pancreatic ribonuclease, ribonuclease T2 and alkaline phosphatase digestion. methyl-3H-labelled-poly(A)-containing RNA isolated from late erythroid cells contain a methyl-labelled fragment resistant to endonuclease and phosphodiesterase II digestion. After digestion with phosphodiesterase I this fragment produces methyl-3 H-labelled nucleotides with the electrophoretic mobility of pm7G and pAm. It is concluded that globin mRNA has the 5' sequences m7G(5')ppp'AmpYpGp ... and m7G(5')pppAmpApGpYp.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/949325-1054511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/949325-1057180,
http://linkedlifedata.com/resource/pubmed/commentcorrection/949325-1128665,
http://linkedlifedata.com/resource/pubmed/commentcorrection/949325-1168101,
http://linkedlifedata.com/resource/pubmed/commentcorrection/949325-163011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/949325-164018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/949325-164293,
http://linkedlifedata.com/resource/pubmed/commentcorrection/949325-165427,
http://linkedlifedata.com/resource/pubmed/commentcorrection/949325-4182399,
http://linkedlifedata.com/resource/pubmed/commentcorrection/949325-4290988,
http://linkedlifedata.com/resource/pubmed/commentcorrection/949325-4321896,
http://linkedlifedata.com/resource/pubmed/commentcorrection/949325-4352910,
http://linkedlifedata.com/resource/pubmed/commentcorrection/949325-4369338,
http://linkedlifedata.com/resource/pubmed/commentcorrection/949325-4421673,
http://linkedlifedata.com/resource/pubmed/commentcorrection/949325-4502410,
http://linkedlifedata.com/resource/pubmed/commentcorrection/949325-4504350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/949325-4737318,
http://linkedlifedata.com/resource/pubmed/commentcorrection/949325-5011971,
http://linkedlifedata.com/resource/pubmed/commentcorrection/949325-5126919,
http://linkedlifedata.com/resource/pubmed/commentcorrection/949325-5276303,
http://linkedlifedata.com/resource/pubmed/commentcorrection/949325-5656814,
http://linkedlifedata.com/resource/pubmed/commentcorrection/949325-5773836
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
155
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
637-44
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:949325-Animals,
pubmed-meshheading:949325-Base Sequence,
pubmed-meshheading:949325-Binding Sites,
pubmed-meshheading:949325-Chromatography,
pubmed-meshheading:949325-Electrophoresis,
pubmed-meshheading:949325-Globins,
pubmed-meshheading:949325-Isoniazid,
pubmed-meshheading:949325-Molecular Weight,
pubmed-meshheading:949325-Nucleic Acid Denaturation,
pubmed-meshheading:949325-Poly A,
pubmed-meshheading:949325-RNA, Messenger,
pubmed-meshheading:949325-Rabbits,
pubmed-meshheading:949325-Ribonucleases
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pubmed:year |
1976
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pubmed:articleTitle |
The nature of the 5'-linked 5' nucleotide sequence at the 5' end of rabbit globin messenger ribonucleic acid.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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