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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
6
|
| pubmed:dateCreated |
1998-4-9
|
| pubmed:abstractText |
Bone marrow dendritic cells (DC) from patients with multiple myeloma (MM) were recently reported to be infected with Kaposi's sarcoma-associated herpesvirus (KSHV). Because immunotherapy strategies using DC are very promising in this disease, we looked for KSHV DNA in clinical-grade DC generated in vitro from MM patients. Adherent apheresis cells from MM patients were maintained for 7 days in clinical-grade X-VIVO 15 culture medium supplemented with granulocyte-macrophage colony-stimulating factor, interleukin-4, or interleukin-13. Tumor necrosis factor alpha was added for the last 2 days. We obtained a cell population with a DC phenotype able to endocytose fluorescein isothiocyanate (FITC)-dextran and efficiently activate resting allogenic T lymphocytes. To detect KSHV DNA, we used polymerase chain reaction (PCR) followed by Southern blotting of PCR product with a sensitivity detecting a few copies of viral DNA. All the PCR were repeated in a blinded fashion three times, on 1 mug and 0.2 mug of genomic DNA, in two different laboratories. Clinical-grade DC from 10 (91%) of 11 patients were not infected with KSHV. The apheresis cells and the purified CD34(+) cells from the same patients were also negative. A very weak PCR band was detected with DC from one patient, but the initial apheresis cells were negative. The detection of KSHV infection in 1 (9%) of 11 MM patients probably represents background seroprevalence. It seems likely that functional and clinical-grade DC from MM patients can safely be used in clinical trials.
|
| pubmed:commentsCorrections | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Granulocyte-Macrophage...,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-13,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0006-4971
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
91
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1852-7
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9490666-Blood Component Removal,
pubmed-meshheading:9490666-Blotting, Southern,
pubmed-meshheading:9490666-Bone Marrow,
pubmed-meshheading:9490666-Cell Separation,
pubmed-meshheading:9490666-Cells, Cultured,
pubmed-meshheading:9490666-Comorbidity,
pubmed-meshheading:9490666-DNA, Neoplasm,
pubmed-meshheading:9490666-DNA, Viral,
pubmed-meshheading:9490666-Dendritic Cells,
pubmed-meshheading:9490666-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:9490666-Hematopoietic Stem Cells,
pubmed-meshheading:9490666-Herpesviridae Infections,
pubmed-meshheading:9490666-Herpesvirus 8, Human,
pubmed-meshheading:9490666-Humans,
pubmed-meshheading:9490666-Interleukin-13,
pubmed-meshheading:9490666-Interleukin-4,
pubmed-meshheading:9490666-Middle Aged,
pubmed-meshheading:9490666-Multiple Myeloma,
pubmed-meshheading:9490666-Polymerase Chain Reaction,
pubmed-meshheading:9490666-Sensitivity and Specificity,
pubmed-meshheading:9490666-Single-Blind Method
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Clinical-grade functional dendritic cells from patients with multiple myeloma are not infected with Kaposi's sarcoma-associated herpesvirus.
|
| pubmed:affiliation |
Institute for Molecular Genetics, CNRS, Montpellier, France.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|