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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1998-3-31
|
| pubmed:abstractText |
T cells play a pivotal role in initiating and orchestrating allergic responses in asthma. The goal of this work was to learn whether ragweed challenge in the lungs alters the T-cell repertoire expressed in the blood and lungs of atopic asthmatics. Analyses of cell numbers, differentials, and T-cell subsets in bronchoalveolar lavage (BAL) fluids showed that ragweed challenge was associated with preferential recruitment of CD4+ T cells into the lungs. A reverse transcriptase-polymerase chain reaction (RT-PCR) was used to amplify T-cell receptor (TCR) gene transcripts from unfractionated, CD4+, and CD8+ T cells in blood and BAL fluids. As judged by RT-PCR, the usage of TCR Valpha and Vbeta gene families in BAL fluids was similar to that in blood. Ragweed challenge did not change the levels of expression of these V gene families. The clonality of T cells was estimated by analyzing the diversity of TCR V-(D)-J junctional region nucleotide lengths associated with each Valpha and Vbeta gene family, using sequencing gel electrophoresis. Most V gene families in blood and BAL fluids were associated with multiple junctional region lengths before and after ragweed challenge, indicating polyclonal expression. Some V gene families were expressed in an oligoclonal manner in unfractionated, CD4+, and CD8+ T cells in BAL fluids before ragweed challenge, as indicated by a few predominant junctional region lengths. The majority of these V gene families became polyclonal after challenge, compatible with polyclonal T-cell influx during inflammation immediately after ragweed challenge. However, some V gene families became oligoclonal or developed a new oligoclonal pattern of junctional region lengths in BAL T cells after ragweed challenge. Surprisingly, this occurred in both CD4+ and CD8+ T cells. In one of these instances, DNA sequencing of Vbeta21 junctional regions in CD8+ T cells confirmed a change from polyclonal to oligoclonal expression after ragweed challenge. These findings show that ragweed challenge is associated with polyclonal influx and oligoclonal activation of both CD4+ and CD8+ T cells in the lungs.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
1044-1549
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
18
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
370-83
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9490655-Adult,
pubmed-meshheading:9490655-Allergens,
pubmed-meshheading:9490655-Animals,
pubmed-meshheading:9490655-Asthma,
pubmed-meshheading:9490655-Bronchoalveolar Lavage Fluid,
pubmed-meshheading:9490655-CD4-Positive T-Lymphocytes,
pubmed-meshheading:9490655-CD8-Positive T-Lymphocytes,
pubmed-meshheading:9490655-Cats,
pubmed-meshheading:9490655-Female,
pubmed-meshheading:9490655-Humans,
pubmed-meshheading:9490655-Hypersensitivity, Immediate,
pubmed-meshheading:9490655-Lung,
pubmed-meshheading:9490655-Male,
pubmed-meshheading:9490655-Mites,
pubmed-meshheading:9490655-Pollen,
pubmed-meshheading:9490655-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:9490655-Sequence Analysis, DNA,
pubmed-meshheading:9490655-T-Lymphocytes
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
T-Cell repertoire in the blood and lungs of atopic asthmatics before and after ragweed challenge.
|
| pubmed:affiliation |
Department of Medicine, University of Maryland, Baltimore, Maryland 21201, USA. vyurovsk@umabnet.ab.umd.edu
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
|