Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1998-3-12
pubmed:abstractText
Induction of the prodynorphin gene occurs in a tissue-specific manner following different physiological stimuli. Using electrophoretic mobility shift assays, we studied the relative activity of the five major regulatory sites in the rat prodynorphin promoter. Prodynorphin cyclic AMP-responsive element 2 (DynCRE2), DynCRE3, and the noncanonical prodynorphin AP-1 (ncDynAP-1) regulatory sites control, in a coordinated manner, prodynorphin induction in the spinal cord after noxious stimulation, whereas prodynorphin up-regulation in supraoptic neurons is regulated predominantly by the ncDynAP-1. Conversely, prodynorphin transactivation in the ovaries upon gonadotropin stimulation is controlled by DynCRE1 and DynCRE3. Our results support the idea that stimulus-specific changes in nuclear proteins establish a functional hierarchy among regulatory sites in the prodynorphin promoter and provide further insight in the molecular mechanisms that govern prodynorphin gene regulation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0022-3042
pubmed:author
pubmed:issnType
Print
pubmed:volume
70
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
914-21
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:9489710-Animals, pubmed-meshheading:9489710-Cell Nucleus, pubmed-meshheading:9489710-Cyclic AMP, pubmed-meshheading:9489710-Cyclic AMP Response Element-Binding Protein, pubmed-meshheading:9489710-Electrophoresis, pubmed-meshheading:9489710-Enhancer Elements, Genetic, pubmed-meshheading:9489710-Enkephalins, pubmed-meshheading:9489710-Female, pubmed-meshheading:9489710-Gene Expression Regulation, pubmed-meshheading:9489710-Gonadotropins, pubmed-meshheading:9489710-Osmotic Pressure, pubmed-meshheading:9489710-Ovary, pubmed-meshheading:9489710-Pain, pubmed-meshheading:9489710-Promoter Regions, Genetic, pubmed-meshheading:9489710-Protein Precursors, pubmed-meshheading:9489710-Rats, pubmed-meshheading:9489710-Rats, Wistar, pubmed-meshheading:9489710-Spinal Cord, pubmed-meshheading:9489710-Stimulation, Chemical, pubmed-meshheading:9489710-Supraoptic Nucleus, pubmed-meshheading:9489710-Transcription, Genetic, pubmed-meshheading:9489710-Transcription Factor AP-1
pubmed:year
1998
pubmed:articleTitle
Stimulus-specific hierarchy of enhancer elements within the rat prodynorphin promoter.
pubmed:affiliation
Instituto Cajal, Consejo Superior de Investigaciones Cientificas, Madrid, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't