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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1998-4-2
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pubmed:abstractText |
Synaptosomal-associated protein of 25 kDa (SNAP-25) is a palmitoylated membrane protein essential for neurotransmitter release from synaptic terminals. We used neuronal cell lines to study the biosynthesis and posttranslational processing of SNAP-25 to investigate how palmitoylation contributes to the subcellular localization of the protein. SNAP-25 was synthesized as a soluble protein that underwent palmitoylation approximately 20 min after synthesis. Palmitoylation of the protein coincided with its stable membrane association. Treatment of cells with brefeldin A or other disrupters of transport inhibited palmitoylation of newly synthesized SNAP-25 and abolished membrane association. These results demonstrate that the processing of SNAP-25 and its targeting to the plasma membrane depend on an intact transport mechanism along the exocytic pathway. The kinetics of SNAP-25 palmitoylation and membrane association and the sensitivity of these parameters to brefeldin A suggest a novel trafficking pathway for targeting proteins to the plasma membrane. In vitro, SNAP-25 stably associated with membranes was not released from the membrane after chemical deacylation. We propose that palmitoylation of SNAP-25 is required for initial membrane targeting of the protein but that other interactions can maintain membrane association in the absence of fatty acylation.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9487128-1281490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9487128-1282335,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9487128-1457777,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9487128-14731773,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9487128-1740466,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9487128-1847146,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9487128-1903488,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9487128-1934055,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9487128-2208277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9487128-2406380,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9487128-2537833,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9487128-2592413,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9487128-2648946,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9487128-2743999,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9487128-2767324,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9487128-2797153,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9487128-6340834,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9487128-6438083,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9487128-7530714,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9487128-7629308,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9487128-7651161,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9487128-7673226,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9487128-7716512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9487128-7791897,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9487128-7822269,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9487128-7835332,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9487128-7893678,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9487128-8007974,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9487128-8106550,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9487128-8197108,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9487128-8221884,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9487128-8399188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9487128-8399217,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9487128-8455717,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9487128-8601435,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9487128-8636152,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9487128-8641455,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9487128-8655574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9487128-9060467
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Brefeldin A,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclopentanes,
http://linkedlifedata.com/resource/pubmed/chemical/GAP-43 Protein,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Neurotransmitter Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Palmitic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Synthesis Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Snap25 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Synaptosomal-Associated Protein 25
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1059-1524
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
585-97
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9487128-Animals,
pubmed-meshheading:9487128-Rats,
pubmed-meshheading:9487128-Cyclopentanes,
pubmed-meshheading:9487128-Palmitic Acids,
pubmed-meshheading:9487128-Kinetics,
pubmed-meshheading:9487128-Membrane Proteins,
pubmed-meshheading:9487128-Protein Conformation,
pubmed-meshheading:9487128-Cell Membrane,
pubmed-meshheading:9487128-Nerve Tissue Proteins,
pubmed-meshheading:9487128-Biological Transport, Active,
pubmed-meshheading:9487128-Subcellular Fractions,
pubmed-meshheading:9487128-Cell Line,
pubmed-meshheading:9487128-Presynaptic Terminals,
pubmed-meshheading:9487128-Neurotransmitter Agents,
pubmed-meshheading:9487128-Protein Synthesis Inhibitors,
pubmed-meshheading:9487128-Acylation,
pubmed-meshheading:9487128-Protein Processing, Post-Translational,
pubmed-meshheading:9487128-GTP-Binding Proteins,
pubmed-meshheading:9487128-Brefeldin A,
pubmed-meshheading:9487128-GAP-43 Protein,
pubmed-meshheading:9487128-PC12 Cells,
pubmed-meshheading:9487128-Synaptosomal-Associated Protein 25
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