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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1998-3-13
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pubmed:databankReference |
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pubmed:abstractText |
Using autoimmune serum from a patient with anti-centromere antibodies, we have identified and partially characterized a novel protein with a mol. wt of approximately 27 kD (hereafter referred to as p27). A cDNA expression library was screened with this serum, and two overlapping inserts were isolated among three positive clones other than clones corresponding to centromere protein (CENP)-B and CENP-C. Analysis of the sequence showed an open reading frame of approximately 0.6 kb encoding 199 amino acids with a predicted mol. wt of 21.5 kD. Immunoblotting analysis with bacterial recombinant p27 showed that approximately 2% of anti-centromere antibody-positive patients had autoantibodies to p27, whereas only one of 215 autoimmune patients without anti-centromere antibodies reacted with the recombinant. All five cases with anti-p27 antibodies, who were diagnosed as having scleroderma and/or Sjögren's syndrome, showed internal organ involvement. Although affinity-purified anti-p27 human or mouse polyclonal antibodies failed to stain any cellular structures in an immunofluorescence study, the potential association of anti-p27 with anti-centromere antibodies suggests that this novel autoantigen might play a role in mitosis.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9486406-1283396,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9486406-1288420,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9486406-1376639,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9486406-1845841,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9486406-2213779,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9486406-2646863,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9486406-3257874,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9486406-3313277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9486406-3511098,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9486406-6337593,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9486406-6966403,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9486406-7539349,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9486406-7864889,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9486406-7883764,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9486406-8069468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9486406-8294433,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9486406-8505380,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9486406-8706335,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9486406-8864858,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9486406-9112222,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9486406-9146917
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Autoantibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Autoantigens,
http://linkedlifedata.com/resource/pubmed/chemical/CENPB protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Cenpb protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Centromere Protein B,
http://linkedlifedata.com/resource/pubmed/chemical/Chromosomal Proteins, Non-Histone,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/centromere protein C
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0009-9104
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
111
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
372-6
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9486406-Aged,
pubmed-meshheading:9486406-Amino Acid Sequence,
pubmed-meshheading:9486406-Animals,
pubmed-meshheading:9486406-Autoantibodies,
pubmed-meshheading:9486406-Autoantigens,
pubmed-meshheading:9486406-Base Sequence,
pubmed-meshheading:9486406-Blotting, Western,
pubmed-meshheading:9486406-Centromere,
pubmed-meshheading:9486406-Centromere Protein B,
pubmed-meshheading:9486406-Chromosomal Proteins, Non-Histone,
pubmed-meshheading:9486406-Cloning, Molecular,
pubmed-meshheading:9486406-DNA, Complementary,
pubmed-meshheading:9486406-DNA-Binding Proteins,
pubmed-meshheading:9486406-Female,
pubmed-meshheading:9486406-Humans,
pubmed-meshheading:9486406-Male,
pubmed-meshheading:9486406-Mice,
pubmed-meshheading:9486406-Middle Aged,
pubmed-meshheading:9486406-Molecular Sequence Data,
pubmed-meshheading:9486406-Sjogren's Syndrome
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pubmed:year |
1998
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pubmed:articleTitle |
cDNA cloning of a novel autoantigen targeted by a minor subset of anti-centromere antibodies.
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pubmed:affiliation |
Department of Dermatology, Nagoya University School of Medicine, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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