Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2 Pt 2
|
| pubmed:dateCreated |
1998-3-17
|
| pubmed:abstractText |
This study describes a technique for the direct daily measurement of arterial blood pressure, sampling of arterial blood, and continuous intravenous infusion in free-moving, conscious, Swiss-Webster mice. Catheters were chronically implanted in the femoral artery and vein, tunneled subcutaneously, exteriorized at the back of the neck in a lightweight tethering spring, and attached to a swivel device at the top of the cage. Time-control experiments (n = 8) demonstrated stable values of mean arterial pressure (MAP, 116 +/- 1 mmHg) and heart rate (HR, 627 +/- 21 beats/min) for up to 35 days after catheter implantation. It was further observed that restraining mice (n = 7) increased MAP by 10 +/- 3 mmHg and HR by 78 +/- 8 beats/min from the values observed under free-moving conditions. To demonstrate the chronic use of the venous catheter, intravenous infusion of NG-nitro-L-arginine methyl ester (L-NAME, 8.6 mg.kg-1.day-1, n = 6) for 5 days significantly increased MAP from 117 +/- 4 to 131 +/- 4 mmHg without altering HR. In a final group of mice (n = 5), oral L-arginine (2% in drinking water) increased plasma arginine concentration from 90 +/- 7 to 131 +/- 17 microM and prevented L-NAME hypertension. These experiments illustrate the feasibility of long-term intravenous infusion, direct arterial blood pressure measurements, and arterial blood sampling in conscious mice.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0002-9513
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
274
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
R564-70
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:9486319-Animals,
pubmed-meshheading:9486319-Arginine,
pubmed-meshheading:9486319-Arteries,
pubmed-meshheading:9486319-Blood Pressure,
pubmed-meshheading:9486319-Blood Pressure Determination,
pubmed-meshheading:9486319-Blood Specimen Collection,
pubmed-meshheading:9486319-Catheters, Indwelling,
pubmed-meshheading:9486319-Drinking,
pubmed-meshheading:9486319-Enzyme Inhibitors,
pubmed-meshheading:9486319-Heart Rate,
pubmed-meshheading:9486319-Infusions, Intravenous,
pubmed-meshheading:9486319-Mice,
pubmed-meshheading:9486319-NG-Nitroarginine Methyl Ester,
pubmed-meshheading:9486319-Nitric Oxide Synthase
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Long-term measurement of arterial blood pressure in conscious mice.
|
| pubmed:affiliation |
Department of Physiology, Medical College of Wisconsin, Milwaukee 53226, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|