Linked Life Data

9486202

Source:http://linkedlifedata.com/resource/pubmed/id/9486202

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2 Pt 1
pubmed:dateCreated
1998-3-23
pubmed:abstractText
In the present study, we investigated the effects of the naturally occurring hormone dehydroepiandrosterone (DHEA) on hypoxic pulmonary vasoconstriction (HPVC) in isolated ferret lungs and on K+ currents in isolated and cultured ferret pulmonary arterial smooth muscle cells (FPSMCs). Severe alveolar hypoxia (3% O2-5% CO2-92% N2) caused an initial increase in pulmonary arterial pressure (Ppa) that was followed by a reversal in pulmonary hypertension. Maintaining alveolar hypoxia caused a sustained secondary increase in Ppa. Pretreating the lungs with the K(+)-channel inhibitor tetraethylammonium (TEA) caused a small increase in baseline Ppa, potentiated HPVC, and prevented the reversal of HPVC during the sustained alveolar hypoxia. Treating the lungs with DHEA caused a near-complete reversal of HPVC in control lungs and in lungs that were pretreated with TEA. DHEA also reversed the KCl-induced increase in Ppa. In FPSMCs, DHEA caused an adenosine 3',5'-cyclic monophosphate- and guanosine 3',5'-cyclic monophosphate-independent increase in activity of the Ca(2+)-activated K+ (KCa) current. In a cell-attached configuration, DHEA caused a mean shift of -22 mV in the voltage-dependent activation of the KCa channel. We conclude that DHEA is a novel KCa-channel opener of the pulmonary vasculature.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0002-9513
pubmed:author
pubmed:issnType
Print
pubmed:volume
274
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
L186-95
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:9486202-Animals, pubmed-meshheading:9486202-Cell Hypoxia, pubmed-meshheading:9486202-Cells, Cultured, pubmed-meshheading:9486202-Cyclic AMP, pubmed-meshheading:9486202-Cyclic GMP, pubmed-meshheading:9486202-Dehydroepiandrosterone, pubmed-meshheading:9486202-Ferrets, pubmed-meshheading:9486202-Ion Channel Gating, pubmed-meshheading:9486202-Large-Conductance Calcium-Activated Potassium Channels, pubmed-meshheading:9486202-Muscle, Smooth, Vascular, pubmed-meshheading:9486202-Potassium Channels, pubmed-meshheading:9486202-Potassium Channels, Calcium-Activated, pubmed-meshheading:9486202-Pulmonary Alveoli, pubmed-meshheading:9486202-Pulmonary Circulation, pubmed-meshheading:9486202-Pulmonary Wedge Pressure, pubmed-meshheading:9486202-Tetraethylammonium, pubmed-meshheading:9486202-Vasoconstriction
pubmed:year
1998
pubmed:articleTitle
Effect of dehydroepiandrosterone on hypoxic pulmonary vasoconstriction: a Ca(2+)-activated K(+)-channel opener.
pubmed:affiliation
Department of Internal Medicine, University of Utah Health Science Center, Salt Lake City 84132, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.