| pubmed-article:9482916 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9482916 | lifeskim:mentions | umls-concept:C0001483 | lld:lifeskim |
| pubmed-article:9482916 | pubmed:issue | 5 | lld:pubmed |
| pubmed-article:9482916 | pubmed:dateCreated | 1998-4-9 | lld:pubmed |
| pubmed-article:9482916 | pubmed:abstractText | Recombinant adenoviruses provide a versatile system for gene expression studies and therapeutic applications. We report herein a strategy that simplifies the generation and production of such viruses. A recombinant adenoviral plasmid is generated with a minimum of enzymatic manipulations, using homologous recombination in bacteria rather than in eukaryotic cells. After transfections of such plasmids into a mammalian packaging cell line, viral production is conveniently followed with the aid of green fluorescent protein, encoded by a gene incorporated into the viral backbone. Homogeneous viruses can be obtained from this procedure without plaque purification. This system should expedite the process of generating and testing recombinant adenoviruses for a variety of purposes. | lld:pubmed |
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| pubmed-article:9482916 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9482916 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9482916 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:9482916 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9482916 | pubmed:month | Mar | lld:pubmed |
| pubmed-article:9482916 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:9482916 | pubmed:author | pubmed-author:YuJJ | lld:pubmed |
| pubmed-article:9482916 | pubmed:author | pubmed-author:VogelsteinBB | lld:pubmed |
| pubmed-article:9482916 | pubmed:author | pubmed-author:POLGG | lld:pubmed |
| pubmed-article:9482916 | pubmed:author | pubmed-author:ZhouSS | lld:pubmed |
| pubmed-article:9482916 | pubmed:author | pubmed-author:KinzlerK WKW | lld:pubmed |
| pubmed-article:9482916 | pubmed:author | pubmed-author:da CostaL TLT | lld:pubmed |
| pubmed-article:9482916 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9482916 | pubmed:day | 3 | lld:pubmed |
| pubmed-article:9482916 | pubmed:volume | 95 | lld:pubmed |
| pubmed-article:9482916 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9482916 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9482916 | pubmed:pagination | 2509-14 | lld:pubmed |
| pubmed-article:9482916 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:9482916 | pubmed:year | 1998 | lld:pubmed |
| pubmed-article:9482916 | pubmed:articleTitle | A simplified system for generating recombinant adenoviruses. | lld:pubmed |
| pubmed-article:9482916 | pubmed:affiliation | The Howard Hughes Medical Institute, 424 North Bond Street, Baltimore, MD 21231, USA. tche@welchlink.welch.jhu.edu | lld:pubmed |
| pubmed-article:9482916 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9482916 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:9482916 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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