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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1998-4-9
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pubmed:abstractText |
Recombinant adenoviruses provide a versatile system for gene expression studies and therapeutic applications. We report herein a strategy that simplifies the generation and production of such viruses. A recombinant adenoviral plasmid is generated with a minimum of enzymatic manipulations, using homologous recombination in bacteria rather than in eukaryotic cells. After transfections of such plasmids into a mammalian packaging cell line, viral production is conveniently followed with the aid of green fluorescent protein, encoded by a gene incorporated into the viral backbone. Homogeneous viruses can be obtained from this procedure without plaque purification. This system should expedite the process of generating and testing recombinant adenoviruses for a variety of purposes.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9482916-1347277,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9482916-1608446,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9482916-1644927,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9482916-2017680,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9482916-3004975,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9482916-3078719,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9482916-6310523,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9482916-6345791,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9482916-6700592,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9482916-7823861,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9482916-8016135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9482916-8287483,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9482916-8289811,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9482916-8352589,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9482916-8388142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9482916-8676512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9482916-8788172,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9482916-8825485,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9482916-886304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9482916-9032314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9482916-9037067
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
3
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pubmed:volume |
95
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2509-14
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9482916-Adenoviridae,
pubmed-meshheading:9482916-Animals,
pubmed-meshheading:9482916-Cell Line,
pubmed-meshheading:9482916-Escherichia coli,
pubmed-meshheading:9482916-Genes, Reporter,
pubmed-meshheading:9482916-Genetic Vectors,
pubmed-meshheading:9482916-Green Fluorescent Proteins,
pubmed-meshheading:9482916-Humans,
pubmed-meshheading:9482916-Kidney,
pubmed-meshheading:9482916-Luminescent Proteins,
pubmed-meshheading:9482916-Mammals,
pubmed-meshheading:9482916-Plasmids,
pubmed-meshheading:9482916-Polymerase Chain Reaction,
pubmed-meshheading:9482916-Recombinant Fusion Proteins,
pubmed-meshheading:9482916-Recombination, Genetic,
pubmed-meshheading:9482916-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:9482916-Transfection
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pubmed:year |
1998
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pubmed:articleTitle |
A simplified system for generating recombinant adenoviruses.
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pubmed:affiliation |
The Howard Hughes Medical Institute, 424 North Bond Street, Baltimore, MD 21231, USA. tche@welchlink.welch.jhu.edu
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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