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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1998-3-27
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pubmed:abstractText |
In murine macrophages, inducible NO synthase II (iNOS or NOS-II) is induced at the transcriptional level by IFN-gamma, alone or synergistically with LPS. We investigated the possible role of reactions of ADP-ribosylation in triggering the signaling pathways involved in NOS-II gene expression. Stimulation with IFN-gamma and/or LPS of RAW 264.7 macrophages, transiently transfected with the NOS-II promoter, was inhibited by ADP-ribosylation inhibitors, indicating that they interfered with the signal(s) responsible for NOS-II gene transcription. We therefore explored the effect of these inhibitors on the activity of IRF-1 and NF kappa B transcription factors known to be involved in NOS-II induction by IFN-gamma and LPS. No effect was observed on IRF-1 activation. However, NF kappa B binding to its target sequence diminished and transfection experiments with an NF kappa B-driven reporter plasmid demonstrating that ADP-ribosylation inhibitors suppressed NF kappa B-dependent promoter activity. These results provide evidence that a step involving ADP-ribosylation is required to activate NF kappa B-mediated gene transcription.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/3-aminobenzamide,
http://linkedlifedata.com/resource/pubmed/chemical/Benzamides,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon Regulatory Factor-1,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Irf1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Niacinamide,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase Type II,
http://linkedlifedata.com/resource/pubmed/chemical/Nos2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Poly(ADP-ribose) Polymerases,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
13
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pubmed:volume |
243
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
451-7
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:9480829-Animals,
pubmed-meshheading:9480829-Benzamides,
pubmed-meshheading:9480829-DNA-Binding Proteins,
pubmed-meshheading:9480829-Enzyme Induction,
pubmed-meshheading:9480829-Enzyme Inhibitors,
pubmed-meshheading:9480829-Genes, Reporter,
pubmed-meshheading:9480829-Interferon Regulatory Factor-1,
pubmed-meshheading:9480829-Interferon-gamma,
pubmed-meshheading:9480829-Lipopolysaccharides,
pubmed-meshheading:9480829-Macrophages,
pubmed-meshheading:9480829-Mice,
pubmed-meshheading:9480829-NF-kappa B,
pubmed-meshheading:9480829-Niacinamide,
pubmed-meshheading:9480829-Nitric Oxide Synthase,
pubmed-meshheading:9480829-Nitric Oxide Synthase Type II,
pubmed-meshheading:9480829-Phosphoproteins,
pubmed-meshheading:9480829-Poly(ADP-ribose) Polymerases,
pubmed-meshheading:9480829-Promoter Regions, Genetic,
pubmed-meshheading:9480829-Transcription, Genetic,
pubmed-meshheading:9480829-Transcription Factors,
pubmed-meshheading:9480829-Transfection
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pubmed:year |
1998
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pubmed:articleTitle |
Inhibitors of ADP-ribosylation impair inducible nitric oxide synthase gene transcription through inhibition of NF kappa B activation.
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pubmed:affiliation |
Unité 365 INSERM Interférons et Cytokines Institut Curie, Paris, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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