Linked Life Data

9480791

Source:http://linkedlifedata.com/resource/pubmed/id/9480791

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1998-4-9
pubmed:abstractText
The Neisseriaceae can acquire iron (Fe) from lactoferrin (Lf) using host-Lf receptors on the bacterial surface. The binding proteins that are proposed to constitute the receptor have been identified by isolation with immobilized Lf. Using CopB-specific monoclonal antibodies and isogenic CopB mutants, we demonstrate that the 84 kDa protein isolated with immobilized human Lf from Moraxella catarrhalis using low stringency conditions is CopB, an 84 kDa membrane-spanning protein with similarities to other TonB-dependent outer membrane proteins. Affinity isolation of Lf receptors from a variety of M. catarrhalis strains using high stringency conditions revealed a 95 kDa protein migrating slightly faster than LbpA on SDS-PAGE in some strains. Convalescent human antisera from patients infected with M. catarrhalis reacted specifically with this protein, but not LbpA. Proteolysis experiments demonstrated that, unlike LbpA, it was rapidly degraded. The 95 kDa protein, but not LbpA, binds labelled Lf after SDS-PAGE and electroblotting, suggesting the 95 kDa protein is LbpB, the homologue of TbpB. This protein comigrates with LbpA in most strains, which may explain why it had not been previously identified.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0882-4010
pubmed:author
pubmed:copyrightInfo
Copyright 1998 Academic Press Limited.
pubmed:issnType
Print
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
89-100
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Biochemical and immunological properties of lactoferrin binding proteins from Moraxella (Branhamella) catarrhalis.
pubmed:affiliation
Department of Microbiology and Infectious Diseases, University of Calgary, Heritage Medical Research Building, 3330-Hospital Drive, Calgary, Alberta, N.W. T2N 4N1, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't