Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1998-3-25
pubmed:databankReference
pubmed:abstractText
Macrosialin is a transmembrane glycoprotein that is highly expressed in macrophages. In the present studies, macrosialin mRNA levels are shown to be markedly up-regulated during macrophage differentiation of bone marrow progenitor cells in response to macrophage colony-stimulating factor and granulocyte-macrophage colony-stimulating factor. To investigate the mechanisms responsible for regulation of macrosialin expression, we have isolated the macrosialin gene and performed an initial analysis of its transcriptional regulatory elements. The macrosialin promoter and 7.0 kilobase pairs of 5'-flanking information direct high levels of reporter gene activity in monocyte/macrophage-like cells, but little or no expression in nonmyeloid cells. This pattern of expression is dependent on regulatory elements located between -7.0 and -2.5 kilobase pairs from the transcriptional start site that exhibit strong enhancer activity in macrophages and repressor activity in nonmyeloid cells. Analysis of the proximal macrosialin promoter indicates that combinatorial interactions between at least four classes of transcriptional activators, including PU.1/Spi-1 and members of the AP-1 family are required for basal promoter function. PU.1/Spi-1 and c-Jun act synergistically to activate the macrosialin promoter in a nonmyeloid cell line, suggesting that combinatorial interactions between these proteins are involved in regulating macrosialin expression during macrophage differentiation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD, http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation..., http://linkedlifedata.com/resource/pubmed/chemical/CD68 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Granulocyte-Macrophage..., http://linkedlifedata.com/resource/pubmed/chemical/Macrophage Colony-Stimulating Factor, http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins, http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-jun, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger, http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators, http://linkedlifedata.com/resource/pubmed/chemical/proto-oncogene protein Spi-1
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
27
pubmed:volume
273
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5389-99
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:9479000-Amino Acid Sequence, pubmed-meshheading:9479000-Animals, pubmed-meshheading:9479000-Antigens, CD, pubmed-meshheading:9479000-Antigens, Differentiation, Myelomonocytic, pubmed-meshheading:9479000-Base Sequence, pubmed-meshheading:9479000-Cell Differentiation, pubmed-meshheading:9479000-Cloning, Molecular, pubmed-meshheading:9479000-Enhancer Elements, Genetic, pubmed-meshheading:9479000-Gene Expression Regulation, Developmental, pubmed-meshheading:9479000-Genes, Reporter, pubmed-meshheading:9479000-Granulocyte-Macrophage Colony-Stimulating Factor, pubmed-meshheading:9479000-Hematopoietic Stem Cells, pubmed-meshheading:9479000-Macrophage Colony-Stimulating Factor, pubmed-meshheading:9479000-Macrophages, pubmed-meshheading:9479000-Membrane Glycoproteins, pubmed-meshheading:9479000-Mice, pubmed-meshheading:9479000-Molecular Sequence Data, pubmed-meshheading:9479000-Mutagenesis, Site-Directed, pubmed-meshheading:9479000-Promoter Regions, Genetic, pubmed-meshheading:9479000-Protein Binding, pubmed-meshheading:9479000-Proto-Oncogene Proteins, pubmed-meshheading:9479000-Proto-Oncogene Proteins c-jun, pubmed-meshheading:9479000-RNA, Messenger, pubmed-meshheading:9479000-Regulatory Sequences, Nucleic Acid, pubmed-meshheading:9479000-Sequence Analysis, DNA, pubmed-meshheading:9479000-Trans-Activators, pubmed-meshheading:9479000-Transcription, Genetic, pubmed-meshheading:9479000-Tumor Cells, Cultured, pubmed-meshheading:9479000-Up-Regulation
pubmed:year
1998
pubmed:articleTitle
The macrosialin promoter directs high levels of transcriptional activity in macrophages dependent on combinatorial interactions between PU.1 and c-Jun.
pubmed:affiliation
Division of Cardiology, Department of Medicine, University of California, San Diego, La Jolla, California 92093-0651, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.