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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1998-4-2
|
| pubmed:abstractText |
The small intestinal epithelium, composed of epithelial cells (EC) and intraepithelial T lymphocytes, is exposed to numerous ingested antigens. Small intestinal EC may act as accessory and/or antigen presenting cells for intestinal T cells, some of which may mature extrathymically and regulate local immunity and tolerance. Since interleukin-7 (IL-7) plays an essential role in T cell maturation and activation, we examined its expression by human small intestinal EC. IL-7 was detected by ELISA in supernatants from 4 of 4 epithelial layer (EpL) cultures. Using RT-PCR, IL-7 mRNA was detected in 4 EpL studied, and two distinct IL-7 transcripts were identified in 3 of the 4. The ratios of the intensities of the larger to the smaller bands varied amongst individuals. Furthermore, the intensity ratios were higher in whole-thickness intestine and lamina propria preparations than in their corresponding EpL. This is the first report of the expression of two IL-7 transcripts in human intestine and of IL-7 secretion by human small intestinal EpL cells. This supports the hypothesis that small intestinal EC may influence differentiation and/or activation of neighboring T cells. The differential expression of the two transcripts may have important implications for immune regulation in the intestinal epithelium.
|
| pubmed:commentsCorrections | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0198-8859
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
58
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
83-90
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9475337-Biopsy,
pubmed-meshheading:9475337-Bone Marrow Cells,
pubmed-meshheading:9475337-Caco-2 Cells,
pubmed-meshheading:9475337-Cell Differentiation,
pubmed-meshheading:9475337-Cell Line,
pubmed-meshheading:9475337-Colon,
pubmed-meshheading:9475337-Electrophoresis, Agar Gel,
pubmed-meshheading:9475337-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:9475337-Epithelial Cells,
pubmed-meshheading:9475337-Gene Expression,
pubmed-meshheading:9475337-Humans,
pubmed-meshheading:9475337-Interleukin-7,
pubmed-meshheading:9475337-Intestine, Small,
pubmed-meshheading:9475337-Leukocytes, Mononuclear,
pubmed-meshheading:9475337-Polymerase Chain Reaction,
pubmed-meshheading:9475337-RNA, Messenger,
pubmed-meshheading:9475337-T-Lymphocytes,
pubmed-meshheading:9475337-Transcription, Genetic
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Human small intestinal epithelial cells secrete interleukin-7 and differentially express two different interleukin-7 mRNA Transcripts: implications for extrathymic T-cell differentiation.
|
| pubmed:affiliation |
Education and Research Centre, St. Vincent's Hospital, Dublin, Ireland. madrigal@svherc.ucd.ie
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|