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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:dateCreated |
1998-2-26
|
| pubmed:abstractText |
Using a plasmid expression system in HeLa cells, we have previously shown that the fusion (F) protein of simian virus 41 (SV-41) induces cell fusion when coexpressed with the haemagglutinin-neuraminidase (HN) protein of human parainfluenza virus type 2 (PIV-2), while the PIV-2 F protein does not induce cell fusion with the SV-41 HN protein. In the present study, we found that the PIV-2 F protein induced extensive cell fusion with the HN protein of mumps virus (MuV), whereas the SV-41 F protein did not. Chimaeric analyses of the F proteins of PIV-2 and SV-41 identified two regions (designated M1 and M2) on the PIV-2 F protein, either of which was necessary for chimaeric F proteins to show fusogenic activity with the MuV HN protein. Subsequently, two additional regions (P1 and P2) were identified on the PIV-2 F protein, both of which were necessary for chimaeric F proteins to prevent induction of cell fusion with the SV-41 HN protein. Consequently, it was proved that a given chimaeric F protein, harbouring regions P1 and P2 together with either of region M1 or M2, induced cell fusion specifically with HN proteins of PIV-2 and MuV, the same as the PIV-2 F protein. Region M2 was located at the membrane proximal end of the PIV-2 F1 ectodomain, while regions P1, M1 and P2 clustered together in the middle of the ectodomain. These regions on the PIV-2 F protein may be involved in a putative functional interaction with HN proteins, which is considered to be a prerequisite for cell fusion.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Feb
|
| pubmed:issn |
0022-1317
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
79 ( Pt 2)
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
279-89
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9472612-Amino Acid Sequence,
pubmed-meshheading:9472612-Animals,
pubmed-meshheading:9472612-Cell Fusion,
pubmed-meshheading:9472612-Cell Line,
pubmed-meshheading:9472612-Cell Membrane,
pubmed-meshheading:9472612-Cercopithecus aethiops,
pubmed-meshheading:9472612-HN Protein,
pubmed-meshheading:9472612-HeLa Cells,
pubmed-meshheading:9472612-Humans,
pubmed-meshheading:9472612-Molecular Sequence Data,
pubmed-meshheading:9472612-Mutagenesis, Site-Directed,
pubmed-meshheading:9472612-Parainfluenza Virus 2, Human,
pubmed-meshheading:9472612-Recombinant Fusion Proteins,
pubmed-meshheading:9472612-Rubulavirus,
pubmed-meshheading:9472612-Sequence Alignment,
pubmed-meshheading:9472612-Sequence Homology, Amino Acid,
pubmed-meshheading:9472612-Transfection,
pubmed-meshheading:9472612-Viral Fusion Proteins
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Identification of regions on the fusion protein of human parainfluenza virus type 2 which are required for haemagglutinin-neuraminidase proteins to promote cell fusion.
|
| pubmed:affiliation |
Department of Microbiology, Mie University School of Medicine, Tsu, Japan. turudome@doc.medic.mie-u.ac.jp
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|