Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1998-3-16
pubmed:abstractText
CD44 has been identified as a membrane-binding partner for ezrin/radixin/moesin (ERM) proteins, plasma membrane/actin filament cross-linkers. ERM proteins, however, are not necessarily colocalized with CD44 in tissues, but with CD43 and ICAM-2 in some types of cells. We found that glutathione-S-transferase fusion proteins with the cytoplasmic domain of CD43 and ICAM-2, as well as CD44, bound to moesin in vitro. The regions responsible for the in vitro binding of CD43 and CD44 to moesin were narrowed down to their juxta-membrane 20-30-amino acid sequences in the cytoplasmic domain. These sequences and the cytoplasmic domain of ICAM-2 (28 amino acids) were all characterized by the positively charged amino acid clusters. When E-cadherin chimeric molecules bearing these positively charged amino acid clusters of CD44, CD43, or ICAM-2 were expressed in mouse L fibroblasts, they were co-concentrated with ERM proteins at microvilli, whereas those lacking these clusters were diffusely distributed on the cell surface. The specific binding of ERM proteins to the juxta-membrane positively charged amino acid clusters of CD44, CD43, and ICAM-2 was confirmed by immunoprecipitation and site-directed mutagenesis. From these findings, we conclude that ERM proteins bind to integral membrane proteins bearing a positively charged amino acid cluster in their juxta-membrane cytoplasmic domain.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-1355095, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-1401904, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-1419062, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-1429901, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-1469058, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-1707055, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-1716182, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-1831124, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-1924289, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-1955455, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-2120593, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-2205394, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-2472072, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-2591371, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-2642392, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-2646308, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-2674140, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-2965006, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-3046603, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-3047011, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-3298422, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-3416359, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-3467321, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-3498123, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-3768962, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-6885906, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-7518464, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-7532110, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-7532174, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-7538138, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-7553859, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-7579708, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-7612961, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-7638219, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-7744951, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-7798316, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-7844168, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-7896873, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-7983158, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-8089177, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-8167019, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-8183953, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-8207064, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-8227193, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-8248180, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-8269991, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-8360275, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-8379998, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-8416983, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-8421057, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-8453669, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-8489015, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-8522586, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-8527459, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-8537411, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-8557751, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-8717043, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-8858161, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-8915779, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-9013673, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-9048483, http://linkedlifedata.com/resource/pubmed/commentcorrection/9472040-9265657
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Glutathione Transferase, http://linkedlifedata.com/resource/pubmed/chemical/Amino Acids, http://linkedlifedata.com/resource/pubmed/chemical/Blood Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins, http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Sialoglycoproteins, http://linkedlifedata.com/resource/pubmed/chemical/Cell Adhesion Molecules, http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Cytoskeletal Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD, http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD43, http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD44, http://linkedlifedata.com/resource/pubmed/chemical/Microfilament Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Cadherins, http://linkedlifedata.com/resource/pubmed/chemical/Spn protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/ICAM2 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/ezrin
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