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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1998-3-24
|
| pubmed:abstractText |
To elucidate the involvement of nitric oxide in spinal nociceptive processing, the correlation of thermal withdrawal latency with nitric oxide synthase-stained neurons in the rat lumbar dorsal horn was analyzed after adjuvant-induced inflammation. From 4 hr through 5 days after subcutaneous injection of complete Freund's adjuvant into the hind paw, a marked thermal hyperalgesia was observed for heat stimulus applied to the affected region. NADPH-diaphorase- and nitric oxide synthase-positive neurons increased significantly in the superficial layers of the dorsal horn ipsilateral to the inflamed hind paw at day 3 of adjuvant-induced inflammation. No change in NADPH-diaphorase-positive neurons was observed at 1 hr and 1 day of adjuvant-induced inflammation. The intravenous administration of N omega-nitro-L-arginine methyl ester (L-NAME, 50 mg/kg), an inhibitor of nitric oxide synthase, significantly blocked the adjuvant-induced thermal hyperalgesia at day 3 of inflammation, but not at day 1; and it had no effect in non-inflamed rats. This anti-hyperalgesic effect of L-NAME at day 3 of inflammation was reversed by the prior administration of L-arginine (600 mg/kg, i.p.), a substrate of nitric oxide synthase. These data suggest that nitric oxide producing neurons in the spinal dorsal horn are involved in maintaining and facilitating the hyperalgesia associated with chronic nociception.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Arginine,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Freund's Adjuvant,
http://linkedlifedata.com/resource/pubmed/chemical/NADPH Dehydrogenase,
http://linkedlifedata.com/resource/pubmed/chemical/NG-Nitroarginine Methyl Ester,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Dec
|
| pubmed:issn |
0021-5198
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
75
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
327-35
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:9469638-Animals,
pubmed-meshheading:9469638-Arginine,
pubmed-meshheading:9469638-Enzyme Inhibitors,
pubmed-meshheading:9469638-Freund's Adjuvant,
pubmed-meshheading:9469638-Hindlimb,
pubmed-meshheading:9469638-Hot Temperature,
pubmed-meshheading:9469638-Hyperalgesia,
pubmed-meshheading:9469638-Immunohistochemistry,
pubmed-meshheading:9469638-Injections, Intravenous,
pubmed-meshheading:9469638-Injections, Subcutaneous,
pubmed-meshheading:9469638-Male,
pubmed-meshheading:9469638-NADPH Dehydrogenase,
pubmed-meshheading:9469638-NG-Nitroarginine Methyl Ester,
pubmed-meshheading:9469638-Neurons,
pubmed-meshheading:9469638-Nitric Oxide,
pubmed-meshheading:9469638-Nitric Oxide Synthase,
pubmed-meshheading:9469638-Rats,
pubmed-meshheading:9469638-Rats, Sprague-Dawley,
pubmed-meshheading:9469638-Spinal Cord
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Nitric oxide in the rat spinal cord in Freund's adjuvant-induced hyperalgesia.
|
| pubmed:affiliation |
Department of Pharmacology, Osaka University Faculty of Dentistry, Suita, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|