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Predicate | Object |
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rdf:type | |
lifeskim:mentions |
umls-concept:C0009017,
umls-concept:C0017262,
umls-concept:C0018282,
umls-concept:C0033684,
umls-concept:C0185117,
umls-concept:C0205314,
umls-concept:C0444669,
umls-concept:C0679622,
umls-concept:C1171362,
umls-concept:C1267092,
umls-concept:C1336986,
umls-concept:C1514562,
umls-concept:C1515670,
umls-concept:C1880389,
umls-concept:C1883204,
umls-concept:C1883221,
umls-concept:C2911684
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pubmed:issue |
8
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pubmed:dateCreated |
1998-3-19
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pubmed:databankReference | |
pubmed:abstractText |
We previously reported the purification of ps20 (Rowley, D. R., Dang, T. D., Larsen, M., Gerdes, M. J., McBride, L., and Lu, B. (1995) J. Biol. Chem. 270, 22058-22065), a urogenital sinus mesenchymal cell secreted protein having growth-inhibitory properties. We report here cloning of the 1.03-kilobase rat ps20 cDNA clone from the PS-1 (adult rat prostate smooth muscle) cDNA library. Partial clones were obtained by nested polymerase chain reaction with degenerate primers, and full-length ps20 cDNA clones were isolated by plaque hybridization. Sequence analysis revealed that ps20 protein contains a WAP-type "four-disulfide core" motif and is a novel member of the WAP signature protein family composed primarily of secreted serine protease inhibitors. Native ps20 immunoprecipitated from smooth muscle cells and recombinant ps20 both resolved on SDS-polyacrylamide gel electrophoresis with apparent molecular mass of 27-29 kDa under reducing conditions and 21-23 kDa under non-reducing conditions, respectively. Stable ps20-transfectant COS-7 cell lines secreted ps20 and were growth-inhibited relative to mock transfectants. In addition, COS-7 and prostate carcinoma PC-3 cells were growth-inhibited by bacterially expressed ps20. Northern analysis indicated differential expression by tissue with highest expression in the heart. Immunohistochemical localization of ps20 protein showed cell-specific expression by both visceral and vascular smooth muscle in all tissues, including the prostate gland. These results indicate ps20 is a novel growth-regulatory member of the WAP signature family expressed by smooth muscle cells.
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pubmed:grant | |
pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Disulfides,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Wfdc1 protein, rat
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0021-9258
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
20
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pubmed:volume |
273
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4574-84
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:9468514-Amino Acid Sequence,
pubmed-meshheading:9468514-Animals,
pubmed-meshheading:9468514-Base Sequence,
pubmed-meshheading:9468514-Cell Differentiation,
pubmed-meshheading:9468514-Cell Division,
pubmed-meshheading:9468514-Cell Line,
pubmed-meshheading:9468514-Cloning, Molecular,
pubmed-meshheading:9468514-DNA, Complementary,
pubmed-meshheading:9468514-Disulfides,
pubmed-meshheading:9468514-Female,
pubmed-meshheading:9468514-Growth Inhibitors,
pubmed-meshheading:9468514-Male,
pubmed-meshheading:9468514-Molecular Sequence Data,
pubmed-meshheading:9468514-Muscle, Smooth,
pubmed-meshheading:9468514-Proteins,
pubmed-meshheading:9468514-Rats,
pubmed-meshheading:9468514-Rats, Sprague-Dawley,
pubmed-meshheading:9468514-Sequence Homology, Amino Acid
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pubmed:year |
1998
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pubmed:articleTitle |
Molecular cloning and expression of ps20 growth inhibitor. A novel WAP-type "four-disulfide core" domain protein expressed in smooth muscle.
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pubmed:affiliation |
Cell and Molecular Biology Program, Baylor College of Medicine, Houston, Texas 77030, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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