Linked Life Data

9468224

Source:http://linkedlifedata.com/resource/pubmed/id/9468224

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1998-2-26
pubmed:abstractText
The round-spotted pufferfish Tetraodon fluviatilis has a genome size of 380 Mb which is slightly smaller than that of another pufferfish Fugu rubripes rubripes (Fugu). Due to its compact genome and small introns, Fugu has been introduced as a model for genome studies. Recently, the round-spotted pufferfish has also been proposed as a new model for genome studies because of the ease in obtaining material and high-sequence homology to that of Fugu. In this study, we have cloned and characterized the snf5 and set genes from the round-spotted pufferfish. The snf5 gene is composed of 9 exons spanning about 2.9 kb whereas the set gene consists of 8 exons spanning about 2.7 kb. They are linked in a tail-to-tail manner with an intergenic region of about 6.5 kb. So far, the genomic structures of human snf5 and set genes are unknown. Based on our data, the pufferfish SNF5 and SET display high amino acid sequence identity (>90%) with the respective human genes. By primer extension and sequence analysis, we found that putative promoter region of the snf5 gene contains a typical TATA box and numerous potential binding sites for transcription factors including AP1, AP2, AP3, c-Myb, HNF-5, and NF-IL6. As for the set gene, its promoter region does not have any TATA or CCAAT motif and contains a few potential binding sites for transcriptional factors such as c-Myb and gamma-IRE. When these promoter regions were placed upstream of the CAT reporter gene and transfected into a carp CF cell line, the 5'-upstream 1.6-kb DNA fragment of the snf5 gene displayed stronger promoter activity, approximately three-fold higher than that of the 5'-upstream 1.3 kb DNA fragment of the set gene. By transient expression and immunofluorescent staining, we also showed that the pufferfish SNF5 and SET are nuclear proteins, consistent with their postulated roles as transcriptional factors.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1044-5498
pubmed:author
pubmed:issnType
Print
pubmed:volume
17
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
69-82
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:9468224-Amino Acid Sequence, pubmed-meshheading:9468224-Animals, pubmed-meshheading:9468224-Base Sequence, pubmed-meshheading:9468224-Chromosomal Proteins, Non-Histone, pubmed-meshheading:9468224-DNA-Binding Proteins, pubmed-meshheading:9468224-Enzyme Inhibitors, pubmed-meshheading:9468224-Exons, pubmed-meshheading:9468224-Fishes, pubmed-meshheading:9468224-Genes, pubmed-meshheading:9468224-Genetic Linkage, pubmed-meshheading:9468224-Histone Chaperones, pubmed-meshheading:9468224-Humans, pubmed-meshheading:9468224-Introns, pubmed-meshheading:9468224-Molecular Sequence Data, pubmed-meshheading:9468224-Phosphoprotein Phosphatases, pubmed-meshheading:9468224-Protein Phosphatase 2, pubmed-meshheading:9468224-Proteins, pubmed-meshheading:9468224-Restriction Mapping, pubmed-meshheading:9468224-Sequence Alignment, pubmed-meshheading:9468224-Sequence Homology, Amino Acid, pubmed-meshheading:9468224-Transcription Factors
pubmed:year
1998
pubmed:articleTitle
Round-spotted pufferfish (Tetraodon fluviatilis) snf5 gene is oriented in a tail-to-tail manner with the set gene which encodes an inhibitor of protein phosphatase 2A.
pubmed:affiliation
Graduate Institute of Life Science, National Defense Medical Center, Taipei, Taiwan.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't