Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1998-3-6
pubmed:abstractText
A rotavirus (RV) nonstructural protein, NSP4, has recently been proposed to function as an enterotoxin in the pathogenesis of RV diarrhea. The role of NSP4 in the pathogenesis of RV diarrhea was examined by infecting cystic fibrosis transmembrane conductance regulator (CFTR) knockout mice with virulent murine RV and by comparing deduced amino acid sequences of RV gene 10 encoding NSP4 from three distinct sets of virulent and tissue culture-adapted avirulent variant RVs. Homozygous CFTR (CFTR-/-) mice, which do not respond to any known intestinal secretagogues, experienced diarrhea comparable to that in normal CFTR+/+ littermates after RV challenge. Comparison of amino acid sequences of NSP4 from virulent and attenuated pairs of RVs failed to show consistent or significant changes. Together, these data suggest that enterotoxigenic properties of RV NSP4 are not critical in the pathogenesis of murine RV diarrhea and that attenuation of murine RVs is not usually mediated by mutations in the gene encoding NSP4.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0022-1899
pubmed:author
pubmed:issnType
Print
pubmed:volume
177
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
455-8
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:9466536-Animals, pubmed-meshheading:9466536-Cells, Cultured, pubmed-meshheading:9466536-Diarrhea, pubmed-meshheading:9466536-Glycoproteins, pubmed-meshheading:9466536-Mice, pubmed-meshheading:9466536-Mice, Inbred C57BL, pubmed-meshheading:9466536-Mice, Inbred CFTR, pubmed-meshheading:9466536-Mice, Knockout, pubmed-meshheading:9466536-Mutation, pubmed-meshheading:9466536-Polymerase Chain Reaction, pubmed-meshheading:9466536-Rotavirus, pubmed-meshheading:9466536-Rotavirus Infections, pubmed-meshheading:9466536-Sequence Alignment, pubmed-meshheading:9466536-Sequence Analysis, DNA, pubmed-meshheading:9466536-Sequence Homology, Amino Acid, pubmed-meshheading:9466536-Toxins, Biological, pubmed-meshheading:9466536-Viral Nonstructural Proteins, pubmed-meshheading:9466536-Virulence
pubmed:year
1998
pubmed:articleTitle
Studies of the role for NSP4 in the pathogenesis of homologous murine rotavirus diarrhea.
pubmed:affiliation
Department of Medicine, Stanford Digestive Disease Center, Stanford University, California, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S., Research Support, Non-U.S. Gov't