Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1998-3-19
pubmed:databankReference
pubmed:abstractText
Photosynthetic membranes of higher plants contain specific nonphosphorous lipids like the sulfolipid sulfoquinovosyl diacylglycerol in addition to the ubiquitous phospholipid phosphatidylglycerol. In bacteria, an environmental factor that drastically affects thylakoid lipid composition appears to be the availability of phosphate. Accordingly, we discovered an increase in the relative amount of sulfolipid and a concomitant decrease in phosphatidylglycerol in Arabidopsis thaliana grown on medium with reduced amounts of phosphate, as well as in the pho1 mutant of A. thaliana deficient in phosphate transport. To investigate the molecular basis of the observed change in lipid composition, we isolated a cDNA of A. thaliana, designated SQD1, that encodes a protein involved in sulfolipid biosynthesis as suggested by three lines of evidence. First, the cDNA shows high sequence similarity to bacterial sqdB genes known to be essential for sulfolipid biosynthesis; second, the SQD1 gene product is imported into chloroplasts where sulfolipid biosynthesis takes place; and third, transgenic plants expressing SQD1 in antisense orientation show a reduction in sulfolipid content. In the pho1 mutant as well as in wild-type plants grown under reduced phosphate availability, increased amounts of SQD1 mRNA and SQD1 protein are detected, suggesting that the increase in sulfolipid content under phosphate limitation is the result of an increased expression of at least one gene required for sulfolipid biosynthesis in A. thaliana. It is suggested that a certain amount of anionic thylakoid lipid is maintained by substituting sulfolipid for phosphatidylglycerol under reduced phosphate availability.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9465123-1400200, http://linkedlifedata.com/resource/pubmed/commentcorrection/9465123-1551852, http://linkedlifedata.com/resource/pubmed/commentcorrection/9465123-16668493, http://linkedlifedata.com/resource/pubmed/commentcorrection/9465123-2231712, http://linkedlifedata.com/resource/pubmed/commentcorrection/9465123-2311769, http://linkedlifedata.com/resource/pubmed/commentcorrection/9465123-2408008, http://linkedlifedata.com/resource/pubmed/commentcorrection/9465123-2441623, http://linkedlifedata.com/resource/pubmed/commentcorrection/9465123-2653818, http://linkedlifedata.com/resource/pubmed/commentcorrection/9465123-4059056, http://linkedlifedata.com/resource/pubmed/commentcorrection/9465123-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/9465123-6276392, http://linkedlifedata.com/resource/pubmed/commentcorrection/9465123-6546423, http://linkedlifedata.com/resource/pubmed/commentcorrection/9465123-7592762, http://linkedlifedata.com/resource/pubmed/commentcorrection/9465123-7846151, http://linkedlifedata.com/resource/pubmed/commentcorrection/9465123-7872771, http://linkedlifedata.com/resource/pubmed/commentcorrection/9465123-7987417, http://linkedlifedata.com/resource/pubmed/commentcorrection/9465123-8000425, http://linkedlifedata.com/resource/pubmed/commentcorrection/9465123-8091210, http://linkedlifedata.com/resource/pubmed/commentcorrection/9465123-8401577, http://linkedlifedata.com/resource/pubmed/commentcorrection/9465123-8434018, http://linkedlifedata.com/resource/pubmed/commentcorrection/9465123-8529635, http://linkedlifedata.com/resource/pubmed/commentcorrection/9465123-8535135, http://linkedlifedata.com/resource/pubmed/commentcorrection/9465123-8631780, http://linkedlifedata.com/resource/pubmed/commentcorrection/9465123-8927627, http://linkedlifedata.com/resource/pubmed/commentcorrection/9465123-9143325
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0027-8424
pubmed:author
pubmed:issnType
Print
pubmed:day
17
pubmed:volume
95
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1950-5
pubmed:dateRevised
2010-9-13
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Phosphate availability affects the thylakoid lipid composition and the expression of SQD1, a gene required for sulfolipid biosynthesis in Arabidopsis thaliana.
pubmed:affiliation
Institut für Genbiologische Forschung Berlin GmbH, Ihnestrasse 63, 14195 Berlin, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't