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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
1998-3-12
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pubmed:abstractText |
Klebsiella oxytoca strain HB60 is highly resistant to cefoperazone and aztreonam (MICs = 128 mg/L). It produces a chromosomally encoded beta-lactamase of pI 5.7 which was highly efficient against penicillins, first-generation cephalosporins and cefoperazone, a non-oxyimino third-generation cephalosporin. Aztreonam and oxyimino broad-spectrum cephalosporins were less good substrates. The beta-lactamase activity was susceptible to inhibition by clavulanic acid (IC50 = 1 microM). The enzyme purified to homogeneity had a specific activity towards benzylpenicillin of 3670 U/mg. The 263 amino acid residues of the protein were sequenced by Edman degradation of proteolytic peptides. The beta-lactamase was shown to belong to the OXY-2 group as it had only one amino acid substitution (Asn for Asp at ABL position 197) compared with the beta-lactamase (pI 5.2) from the aztreonam-susceptible K. oxytoca strain SL911 and two substitutions (Ala223 for Val and Asp255 for Asn) compared with the beta-lactamase (pI 6.4) from the aztreonam-resistant K. oxytoca strain D488. These three OXY-2-group enzymes behave in the same way towards beta-lactam antibiotics. The variability in the resistance of these K. oxytoca strains would thus seem to be due to variation in the level of production of the beta-lactamases rather than to structural alteration of the enzymes.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Aztreonam,
http://linkedlifedata.com/resource/pubmed/chemical/Cephalosporins,
http://linkedlifedata.com/resource/pubmed/chemical/Monobactams,
http://linkedlifedata.com/resource/pubmed/chemical/Penicillins,
http://linkedlifedata.com/resource/pubmed/chemical/beta-Lactamases
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0305-7453
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
40
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
789-95
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9462429-Amino Acid Sequence,
pubmed-meshheading:9462429-Aztreonam,
pubmed-meshheading:9462429-Cephalosporins,
pubmed-meshheading:9462429-Drug Resistance, Microbial,
pubmed-meshheading:9462429-Klebsiella,
pubmed-meshheading:9462429-Molecular Sequence Data,
pubmed-meshheading:9462429-Monobactams,
pubmed-meshheading:9462429-Penicillins,
pubmed-meshheading:9462429-Sequence Homology, Amino Acid,
pubmed-meshheading:9462429-beta-Lactamases
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pubmed:year |
1997
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pubmed:articleTitle |
Characterization and amino acid sequence of the OXY-2 group beta-lactamase of pI 5.7 isolated from aztreonam-resistant Klebsiella oxytoca strain HB60.
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pubmed:affiliation |
Laboratoire de Chimie, URA 401, IFR 63 CNRS, Muséum National d'Histoire Naturelle, Paris, France.
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pubmed:publicationType |
Journal Article,
Comparative Study
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