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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1998-4-9
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pubmed:abstractText |
To investigate the molecular basis of endothelial cell-specific gene expression, we have examined the DNA sequences and the cognate DNA-binding proteins that mediate transcription of the murine tie2/tek gene. Reporter transfection experiments conformed with earlier findings in transgenic mice, indicating that the upstream promoter of Tie2/Tek is capable of activating transcription in an endothelial cell-specific fashion. These experiments have also allowed the identification of a single upstream inhibitory region (region I) and two positive regulatory regions (regions U and A) in the proximal promoter. Electrophoretic mobility-shift assays have allowed further characterization of three novel DNA-binding sequences associated with these regions and have provided preliminary characterization of the protein factors binding to these elements. Two of the elements (U and A) confer increased transcription on a heterologous promoter, with element U functioning in an endothelial-cell-selective manner. By employing embryonic endothelial-like yolk sac cells in parallel with adult-derived endothelial cells, we have identified differences in functional activity and protein binding that may reflect mechanisms for specifying developmental regulation of tie2/tek expression. Further study of the DNA and protein elements characterized in these experiments is likely to provide new insight into the molecular basis of developmental- and cell-specific gene expression in the endothelium.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9461528-1328870,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9461528-1714909,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9461528-1763820,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9461528-1840554,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9461528-2191950,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9461528-2192642,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9461528-2216722,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9461528-2304915,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9461528-2478056,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9461528-2612361,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9461528-3048971,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9461528-3308401,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9461528-3461465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9461528-6828386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9461528-7499271,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9461528-7559454,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9461528-7596435,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9461528-7596436,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9461528-7596437,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9461528-7622033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9461528-7655012,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9461528-7673179,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9461528-7682011,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/9461528-7753844,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/9461528-7958846,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9461528-7958865,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9461528-8041747,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9461528-8599939,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9461528-8608939,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9461528-8632009
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
330 ( Pt 1)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
335-43
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:9461528-Animals,
pubmed-meshheading:9461528-Base Sequence,
pubmed-meshheading:9461528-Cattle,
pubmed-meshheading:9461528-DNA Footprinting,
pubmed-meshheading:9461528-DNA-Binding Proteins,
pubmed-meshheading:9461528-Endothelium, Vascular,
pubmed-meshheading:9461528-Gene Expression Regulation,
pubmed-meshheading:9461528-Mice,
pubmed-meshheading:9461528-Mice, Transgenic,
pubmed-meshheading:9461528-Molecular Sequence Data,
pubmed-meshheading:9461528-Promoter Regions, Genetic,
pubmed-meshheading:9461528-Rats,
pubmed-meshheading:9461528-Receptor, TIE-2,
pubmed-meshheading:9461528-Receptor Protein-Tyrosine Kinases,
pubmed-meshheading:9461528-Restriction Mapping,
pubmed-meshheading:9461528-Transcription, Genetic,
pubmed-meshheading:9461528-Transfection
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pubmed:year |
1998
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pubmed:articleTitle |
Functional analysis of the endothelial cell-specific Tie2/Tek promoter identifies unique protein-binding elements.
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pubmed:affiliation |
Division of Cardiology, Vanderbilt University Medical Center, 315 MRB II, Nashville, TN 37232-6300, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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