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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1998-3-19
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pubmed:abstractText |
Renal interstitial scarring is an important component of heroin-associated nephropathy. Kidney fibroblasts have been demonstrated to play a role in the development of renal scarring in a variety of renal diseases. We studied the effect of morphine, an active metabolite of heroin, on the proliferation of kidney fibroblasts. Morphine at a concentration of 10(-12) M enhanced (P < 0.001) the proliferation of kidney fibroblasts (control, 67.5 +/- 2.0 vs. morphine, 112.2 +/- 10.1 x 10(4) cells/well). [3H]thymidine incorporation studies further confirmed these results. Morphine at concentrations of 10(-12) M to 10(-10) M also modulated mRNA expression of early growth related genes (c-fos, c-jun and c-myc). Morphine at concentrations of 10(-8) to 10(-4) M promoted apoptosis of kidney fibroblasts and also enhanced the synthesis of p53 by kidney fibroblasts. We speculate that morphine-induced kidney fibroblast proliferation may be mediated through the activation of early growth related genes, whereas morphine induced kidney fibroblast apoptosis may be mediated through the generation of p53. The present in vitro study provides a hypothetical basis for the role of morphine in the development of renal interstitial scarring in patients with heroin-associated nephropathy.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Benzimidazoles,
http://linkedlifedata.com/resource/pubmed/chemical/Biotin,
http://linkedlifedata.com/resource/pubmed/chemical/Bromodeoxyuridine,
http://linkedlifedata.com/resource/pubmed/chemical/Deoxyuracil Nucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/HOE 33342,
http://linkedlifedata.com/resource/pubmed/chemical/Morphine,
http://linkedlifedata.com/resource/pubmed/chemical/Narcotics,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-fos,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-jun,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-myc,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Suppressor Protein p53
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0085-2538
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
53
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
350-7
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:9461094-Animals,
pubmed-meshheading:9461094-Apoptosis,
pubmed-meshheading:9461094-Benzimidazoles,
pubmed-meshheading:9461094-Biotin,
pubmed-meshheading:9461094-Bromodeoxyuridine,
pubmed-meshheading:9461094-Cell Division,
pubmed-meshheading:9461094-Cells, Cultured,
pubmed-meshheading:9461094-DNA Fragmentation,
pubmed-meshheading:9461094-Deoxyuracil Nucleotides,
pubmed-meshheading:9461094-Fibroblasts,
pubmed-meshheading:9461094-Fluorescent Dyes,
pubmed-meshheading:9461094-Gene Expression,
pubmed-meshheading:9461094-Kidney,
pubmed-meshheading:9461094-Morphine,
pubmed-meshheading:9461094-Narcotics,
pubmed-meshheading:9461094-Proto-Oncogene Proteins c-fos,
pubmed-meshheading:9461094-Proto-Oncogene Proteins c-jun,
pubmed-meshheading:9461094-Proto-Oncogene Proteins c-myc,
pubmed-meshheading:9461094-RNA, Messenger,
pubmed-meshheading:9461094-Rats,
pubmed-meshheading:9461094-Staining and Labeling,
pubmed-meshheading:9461094-Tumor Suppressor Protein p53
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pubmed:year |
1998
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pubmed:articleTitle |
Morphine modulates proliferation of kidney fibroblasts.
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pubmed:affiliation |
Department of Medicine, Long Island Jewish Medical Center, New Hyde Park, New York 11040, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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