Linked Life Data

9460762

Source:http://linkedlifedata.com/resource/pubmed/id/9460762

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1998-3-12
pubmed:abstractText
Neurons and glial cells are capable of synthesizing various bioactive steroids, but the neuronal mechanisms controlling neurosteroid-secreting cells are poorly understood. In the present study, we have investigated the possible effect of an endogenous ligand of benzodiazepine receptors, the triakontatetraneuropeptide [17-50] (TTN), on steroid biosynthesis in the frog hypothalamus. Immunohistochemical studies revealed that most hypothalamic neurons expressing 3 beta-hydroxysteroid dehydrogenase/delta 5-delta 4-isomerase also contained peripheral-type benzodiazepine receptor-like immunoreactivity. Confocal laser scanning microscopic analysis revealed that the peripheral-type benzodiazepine receptor-immunoreactive material was located both in the cytoplasm and at the periphery of the cell bodies. By using the pulse-chase technique, TTN was found to stimulate the conversion of [3H]pregnenolone into various steroids, including 17-hydroxypregnenolone, 5 alpha-dihydrotestosterone and 17-hydroxyprogesterone, in a dose-dependent manner. The peripheral-type benzodiazepine receptor agonist Ro5-4864 mimicked the stimulatory effect of TTN on the formation of neurosteroids. The peripheral-type benzodiazepine receptor antagonist PK11195 significantly reduced the effect of TTN on neurosteroid synthesis, while the central-type benzodiazepine receptor antagonist flumazenil did not affect the formation of neurosteroids evoked by TTN. These data indicate that TTN stimulates the biosynthesis of 3-keto-17 alpha-hydroxysteroids in frog hypothalamic neurons through activation of peripheral-type benzodiazepine receptors likely located at the plasma membrane level.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0306-4522
pubmed:author
pubmed:issnType
Print
pubmed:volume
83
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
555-70
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:9460762-3-Hydroxysteroid Dehydrogenases, pubmed-meshheading:9460762-Animals, pubmed-meshheading:9460762-Benzodiazepinones, pubmed-meshheading:9460762-Chromatography, High Pressure Liquid, pubmed-meshheading:9460762-Convulsants, pubmed-meshheading:9460762-Fluorescent Antibody Technique, Direct, pubmed-meshheading:9460762-GABA-A Receptor Antagonists, pubmed-meshheading:9460762-Hypothalamus, pubmed-meshheading:9460762-Immunohistochemistry, pubmed-meshheading:9460762-Male, pubmed-meshheading:9460762-Neuropeptides, pubmed-meshheading:9460762-Peptide Fragments, pubmed-meshheading:9460762-Peripheral Nerves, pubmed-meshheading:9460762-Rana ridibunda, pubmed-meshheading:9460762-Receptors, GABA-A, pubmed-meshheading:9460762-Steroids, pubmed-meshheading:9460762-Stimulation, Chemical
pubmed:year
1998
pubmed:articleTitle
The endozepine triakontatetraneuropeptide diazepam-binding inhibitor [17-50] stimulates neurosteroid biosynthesis in the frog hypothalamus.
pubmed:affiliation
European Institute for Peptide Research (IFRMP no 23), Laboratory of Cellular and Molecular Neuroendocrinology, INSERM U 413, UA CNRS, University of Rouen, Mont-Saint-Aignan, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't