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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1998-3-6
|
| pubmed:abstractText |
It has been suggested that prolonged formalin fixation and block storage adversely affect hepatitis C virus (HCV) ribonucleic acid (RNA) detection in tissue by reverse transcriptase-polymerase chain reaction (RT-PCR). We attempted to determine whether short-term perfusion fixation (3-5 days) or prolonged formalin storage adversely affects the detection of HCV RNA in paraffin-embedded tissue in comparison with 24-h fixation. Also, we examined the effects of prolonged storage of paraffin blocks on the sensitivity for HCV detection. We performed RT-PCR in formalin-fixed explanted livers from 20 liver allograft recipients known to be HCV positive (10 with specimens stored for 2-4 years and 10 with specimens stored for > 4 years). We compared the results of perioperative needle liver biopsy specimens fixed overnight with liver sections fixed by perfusion for 3-5 days and bulk liver tissue stored in formalin for years (mean, 6.25 years; range, 2-11 years). HCV RNA was detected in 100%, 85%, and 0% of specimens fixed for 24 h, 3-4 days, and years, respectively. We conclude that HCV can be readily detected in tissue fixed by formalin overnight, sensitivity decreases slightly with intermediate-length fixation, and HCV is rendered undetectable by prolonged fixation. In addition, retention of formalin-fixed tissue in paraffin blocks does not affect the sensitivity of HCV detection.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
1052-9551
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
6
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
277-81
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:9458386-Formaldehyde,
pubmed-meshheading:9458386-Hepacivirus,
pubmed-meshheading:9458386-Humans,
pubmed-meshheading:9458386-Liver,
pubmed-meshheading:9458386-Polymerase Chain Reaction,
pubmed-meshheading:9458386-Protein Biosynthesis,
pubmed-meshheading:9458386-RNA, Viral,
pubmed-meshheading:9458386-Sensitivity and Specificity,
pubmed-meshheading:9458386-Time Factors,
pubmed-meshheading:9458386-Tissue Fixation
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Effects of formalin fixation and prolonged block storage on detection of hepatitis C virus RNA in liver tissue.
|
| pubmed:affiliation |
Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905, USA.
|
| pubmed:publicationType |
Journal Article
|