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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1998-3-23
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pubmed:databankReference |
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pubmed:abstractText |
We have cloned and characterized the Na,K-ATPase beta 3 subunit gene (ATP1B3), and a beta 3 subunit pseudogene (ATP1B3P1), from a human PAC genomic library. The beta 3 subunit gene is > 50 kb in size and is split into 7 exons. The exon/intron organization of the beta 3 subunit gene is identical to that of the Na,K-ATPase beta 3 subunit gene, indicating that these two genes evolved from a common evolutionary ancestor. Comparison of the promoter region of the human and mouse beta 3 subunit gene reveals a high degree of homology within a 300-bp segment located immediately upstream of the translation start site, suggesting that control elements that serve to regulate the cell-specific expression of the beta 3 subunit gene are likely to be located within this conserved region. Dot blot analysis of beta 3 subunit transcripts revealed expression within virtually all human tissues, while in situ hybridization showed expression of beta 3 mRNA in both neurons and glia of rat brain. Fluorescence in situ hybridization with PAC DNA clones localized ATP1B3 to the q22-->23 region of Chromosome (Chr) 3, and the beta 3 pseudogene to the p13-->15 region of Chr 2.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0938-8990
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
9
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
136-43
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:9457675-Amino Acid Sequence,
pubmed-meshheading:9457675-Animals,
pubmed-meshheading:9457675-Base Sequence,
pubmed-meshheading:9457675-Chromosome Mapping,
pubmed-meshheading:9457675-Chromosomes, Human, Pair 2,
pubmed-meshheading:9457675-Chromosomes, Human, Pair 3,
pubmed-meshheading:9457675-Cloning, Molecular,
pubmed-meshheading:9457675-Exons,
pubmed-meshheading:9457675-Gene Expression,
pubmed-meshheading:9457675-Gene Library,
pubmed-meshheading:9457675-Humans,
pubmed-meshheading:9457675-In Situ Hybridization, Fluorescence,
pubmed-meshheading:9457675-Introns,
pubmed-meshheading:9457675-Mice,
pubmed-meshheading:9457675-Molecular Sequence Data,
pubmed-meshheading:9457675-Promoter Regions, Genetic,
pubmed-meshheading:9457675-Pseudogenes,
pubmed-meshheading:9457675-Rats,
pubmed-meshheading:9457675-Sodium-Potassium-Exchanging ATPase,
pubmed-meshheading:9457675-Transcription, Genetic
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pubmed:year |
1998
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pubmed:articleTitle |
Structural organization and chromosomal localization of the human Na,K-ATPase beta 3 subunit gene and pseudogene.
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pubmed:affiliation |
Department of Pharmacology, Penn State College of Medicine, Milton S. Hershey Medical Center, Pennsylvania 17033, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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